Literature summary for 4.2.2.23 extracted from

McKie, V.A.; Vincken, J.P.; Voragen, A.G.; van den Broek, L.A.; Stimson, E.; Gilbert, H.J.
A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose (2001), Biochem. J., 355, 167-177.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Expression of the three modules of the Pseudomonas protein in Escherichia coli shows that its C-terminal module is a functional cellulose-binding domain, and the N-terminal module consists of a catalytic domain that hydrolyzes rhamnogalacturonan-containing substrates	Cellvibrio japonicus

General Stability

General Stability	Organism
completely resistant to proteinase attack	Cellvibrio japonicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	potato pectic galactan	KM-value: 8.5 mg/ml at pH 9.5	Cellvibrio japonicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	absolute requirement for calcium	Cellvibrio japonicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
93415	-	x * 93415, calculated from sequence	Cellvibrio japonicus

Organism

Organism	UniProt	Comment	Textmining
Cellvibrio japonicus	Q9AF09	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
potato pectic galactan	apple-derived and potato-derived rhamnogalacturonan substrates. The enzyme has a strong preference for rhamnogalacturonans that contain galactose side chains, and which are not esterified. The increase in absorbance at 235 nm indicates that glycosidic bond cleavage is mediated via a beta-elimination mechanism	Cellvibrio japonicus	potato pectic galactan oligosaccharides	-	?
rhamnogalacturonan I	apple-derived and potato-derived rhamnogalacturonan substrates. The enzyme has a strong preference for rhamnogalacturonans that contain galactose side chains, and which are not esterified. The increase in absorbance at 235 nm indicates that glycosidic bond cleavage is mediated via a beta-elimination mechanism	Cellvibrio japonicus	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?

Subunits

Subunits	Comment	Organism
?	x * 93415, calculated from sequence	Cellvibrio japonicus

Synonyms

Synonyms	Comment	Organism
Rgl11A	-	Cellvibrio japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	10 min, about 5% loss of activity	Cellvibrio japonicus
60	-	10 min, about 40% loss of activity	Cellvibrio japonicus
65	-	10 min, about 65% loss of activity	Cellvibrio japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	potato pectic galactan	kcat-value: 6500/s at pH 9.5	Cellvibrio japonicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Cellvibrio japonicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.5	10.5	pH 8.5: about 60% of maximal activity, pH 10.5: about about 40% of maximal activity	Cellvibrio japonicus

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Release 2023.1 (January 2023)