Literature summary for 4.2.2.23 extracted from

Ochiai, A.; Itoh, T.; Kawamata, A.; Hashimoto, W.; Murata, K.
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization (2007), Appl. Environ. Microbiol., 73, 3803-3813.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Bacillus subtilis strain natto	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	rhamnogalacturonan I	Km-value is 0.35 mg/ml, pH 7.5, 30°C	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Bacillus subtilis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	1 mM, addition the chelator-treated enzyme restores activity	Bacillus subtilis
Co2+	1 mM, addition the chelator-treated enzyme restores activity	Bacillus subtilis
Mn2+	1 mM, addition the chelator-treated enzyme restores activity	Bacillus subtilis
Zn2+	1 mM, addition the chelator-treated enzyme restores activity	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64444	-	1 * 64444, mature form of YesW including the C-terminal 8His-tag	Bacillus subtilis
65000	-	gel filtration	Bacillus subtilis
68000	-	1 * 68000, SDS-PAGE	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
rhamnogalacturonan I	Bacillus subtilis	the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?
rhamnogalacturonan I	Bacillus subtilis 168	the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O31526	-	-
Bacillus subtilis 168	O31526	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli cells	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
rhamnogalacturonan I	the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR	Bacillus subtilis	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?
rhamnogalacturonan I	rhamnogalacturonan I from potatoes. YesW acts on the substrate endolytically and releases both disaccharide and larger saccharides. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan	Bacillus subtilis	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?
rhamnogalacturonan I	the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR	Bacillus subtilis 168	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?
rhamnogalacturonan I	rhamnogalacturonan I from potatoes. YesW acts on the substrate endolytically and releases both disaccharide and larger saccharides. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan	Bacillus subtilis 168	rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 68000, SDS-PAGE	Bacillus subtilis
monomer	1 * 64444, mature form of YesW including the C-terminal 8His-tag	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Bacillus subtilis
65	-	-	Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	75	40°C: about 45% of maximal activity, 75°C: about 50% of maximal activity	Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	5 min, stable below	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Bacillus subtilis

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	pH 7.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity	Bacillus subtilis

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Release 2023.1 (January 2023)