Literature summary for 4.2.2.22 extracted from

Parisot, J.; Ghochikyan, A.; Langlois, V.; Sakanyan, V.; Rabiller, C.
Exopolygalacturonate lyase from Thermotoga maritima Cloning, characterization and organic synthesis application (2002), Carbohydr. Res., 337, 1427-1433 .

Search for more literature for 4.2.2.22

Application

Application	Comment	Organism
synthesis	the highly thermostable enzyme constitutes a useful catalyst for a simplified synthesis of (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid which is extremely difficult to obtain via chemical synthesis	Thermotoga maritima

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli	Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
EGTA	-	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WYR4	-	-
Thermotoga maritima DSM 3109	Q9WYR4	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	precursor undergoes N-terminal processing by cleavage at a putative site between alanine and serine residues	Thermotoga maritima

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonic acid	the enzyme catalyzes selectively a x01-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid with a 60% yield	Thermotoga maritima	(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid	-	?
polygalacturonic acid	the enzyme catalyzes selectively a x01-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid with a 60% yield	Thermotoga maritima DSM 3109	(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 42000, SDS-PAGE	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
Pel	-	Thermotoga maritima
Tm0433	-	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	the enzyme loses 50% of its initial activity within 30 min	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Thermotoga maritima

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.5	10	pH 8.5: about 70% of maximal activity, pH 10.0: about 50% of maximal activity. Below pH 7 or above pH 11, no appreciable activity can be detected	Thermotoga maritima

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Release 2023.1 (January 2023)