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Literature summary for 4.2.2.2 extracted from
- The purification and characterization of a novel alkali-stable pectate lyase produced by Bacillus subtilis PB1 (2017), World J. Microbiol. Biotechnol., 33, 190 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 77% of initial activity | Bacillus subtilis |  |
| EDTA | 1 mM, 17% of initial activity | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Polygalacturonate | Km value 0.312 mg/ml, pH 9.4, 50°C | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, 139% of initial activity | Bacillus subtilis | |
| Ca2+ | 1 mM, 257% of initial activity | Bacillus subtilis | |
| Mg2+ | 1 mM, 144% of initial activity | Bacillus subtilis | |
| Mn2+ | 1 mM, 143% of initial activity | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | A0A286K338 | - |
- |
| Bacillus subtilis PB1 | A0A286K338 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1252 | - |
pH 9.4, 50°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin | - |
Bacillus subtilis | unsaturated oligogalacturonate | - |
? | |
| citrus pectin | - |
Bacillus subtilis PB1 | unsaturated oligogalacturonate | - |
? | |
| polygalacturonate | - |
Bacillus subtilis | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | - |
Bacillus subtilis PB1 | unsaturated oligogalacturonides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43100, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | 2 h, 90% residual activity | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2300 | - |
Polygalacturonate | pH 9.4, 50°C | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | 2 h, 80% residual activity | Bacillus subtilis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Polygalacturonate | kcat/Km value 7372 ml/mg/s, pH 9.4, 50°C | Bacillus subtilis | |
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