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Literature summary for 4.2.2.2 extracted from
- Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3) (2015), Protein Expr. Purif., 110, 43-51 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | presence of Ca2+ stimulates a change in tertiary structure. Binding of Ca2+ and polygalacturonic acid to the active site may occur independently | Bacillus subtilis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | I0BWH3 | - |
- |
| Bacillus subtilis RCK | I0BWH3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, both from soluble fraction and from inclusion bodies | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1493 | - |
recombinant protein purified from inclusion bodies, pH 9.0, 50°C | Bacillus subtilis |
| 3194 | - |
recombinant protein purified from soluble fraction, pH 9.0, 50°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin | - |
Bacillus subtilis | unsaturated oligogalacturonides | - |
? | |
| citrus pectin | - |
Bacillus subtilis RCK | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | - |
Bacillus subtilis | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | - |
Bacillus subtilis RCK | unsaturated oligogalacturonides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45470, MALDI-TOF | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C0W65_13990 | locus name | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life 55 min, recombinant protein | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | more than 80% of maximum activity | Bacillus subtilis |
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