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Literature summary for 4.2.2.2 extracted from

  • Saoudi, B.; Habbeche, A.; Kerouaz, B.; Haberra, S.; Ben Romdhane, Z.; Tichati, L.; Boudelaa, M.; Belghith, H.; Gargouri, A.; Ladjama, A.
    Purification and characterization of a new thermoalkaliphilic pectate lyase from Actinomadura keratinilytica Cpt20 (2015), Process Biochem., 50, 2259-2266 .
No PubMed abstract available
Search for more literature for 4.2.2.2

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, no residual activity Actinomadura keratinilytica
Cu2+ 1 mM, 51% of initial activity Actinomadura keratinilytica
EDTA complete inhibition Actinomadura keratinilytica
Hg2+ 1 mM, no residual activity Actinomadura keratinilytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 Polygalacturonate pH 9.0, 55°C Actinomadura keratinilytica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Actinomadura keratinilytica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 216% of initial activity Actinomadura keratinilytica
Ca2+ 1 mM, 306% of initial activity. Presence of Ca2+ enhances thermoactivity and thermostability Actinomadura keratinilytica
Co2+ 1 mM, 180% of initial activity Actinomadura keratinilytica
Fe2+ 1 mM, 126% of initial activity Actinomadura keratinilytica
Mg2+ 1 mM, 226% of initial activity Actinomadura keratinilytica
Mn2+ 1 mM, 268% of initial activity Actinomadura keratinilytica
Zn2+ 1 mM, 202% of initial activity Actinomadura keratinilytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 gel filtration Actinomadura keratinilytica

Organism

Organism UniProt Comment Textmining
Actinomadura keratinilytica
-
-
-
Actinomadura keratinilytica Cpt20
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Actinomadura keratinilytica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21889
-
 pH 9.0, 55°C Actinomadura keratinilytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information best substrate: low-esterified pectin Actinomadura keratinilytica ?
-
 ?
additional information best substrate: low-esterified pectin Actinomadura keratinilytica Cpt20 ?
-
 ?
pectin 32% esterification, best substrate. At 54% esterification, about 70% of the activity with pectin of 32% esterification Actinomadura keratinilytica unsaturated oligogalacturonides
-
 ?
pectin 32% esterification, best substrate. At 54% esterification, about 70% of the activity with pectin of 32% esterification Actinomadura keratinilytica Cpt20 unsaturated oligogalacturonides
-
 ?
polygalacturonate about 75% of the activity with pectin of 32% esterification Actinomadura keratinilytica unsaturated oligogalacturonides
-
 ?
polygalacturonate about 75% of the activity with pectin of 32% esterification Actinomadura keratinilytica Cpt20 unsaturated oligogalacturonides
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE, 1 * 34125, MALDI-TOF Actinomadura keratinilytica

Synonyms

Synonyms Comment Organism
Pel-20
-
 Actinomadura keratinilytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Actinomadura keratinilytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life 18 h Actinomadura keratinilytica
80
-
 half-life 12 h Actinomadura keratinilytica
90
-
 half-life 7 h Actinomadura keratinilytica
100
-
 half-life 2 h Actinomadura keratinilytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Actinomadura keratinilytica

pH Range

pH Minimum pH Maximum Comment Organism
7.5 12
-
 Actinomadura keratinilytica