Protein Variants | Comment | Organism |
---|---|---|
I140V | about 25% decrease in catalytic efficiency | Aspergillus luchuensis var. saitoi |
V197D | about 25% decrease in catalytic efficiency | Aspergillus luchuensis var. saitoi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5.0 mM, 30-35% inhibition | Aspergillus luchuensis var. saitoi | |
Fe3+ | 5.0 mM, 30-35% inhibition | Aspergillus luchuensis var. saitoi | |
additional information | not inhibitory: 0.5% SDS, 5% Tween-20, 10% Triton X-100, 5% acetone, 20% ethanol, 10% isopropanol, and 10% methanol | Aspergillus luchuensis var. saitoi | |
Zn2+ | 5.0 mM, 30-35% inhibition | Aspergillus luchuensis var. saitoi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Polygalacturonate | Km value of wild-type, 0.74 mg/ml, of mutant I140V 1.07 mg/ml, of mutant V197D 0.71 mg/ml, respectively, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
additional information | - |
citrus peel pectin | Km value of wild-type, 0.97 mg/ml, of mutant I140V 0.93 mg/ml, of mutant V197D 0.91 mg/ml, respectively, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
additional information | - |
apple pectin | Km value of wild-type, 1.12 mg/ml, of mutant I140V 0.97 mg/ml, of mutant V197D 1.00 mg/ml, respectively, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, optimum concentration 0.3 mM | Aspergillus luchuensis var. saitoi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus luchuensis var. saitoi | - |
- |
- |
Aspergillus luchuensis var. saitoi KBN 2022 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apple pectin | - |
Aspergillus luchuensis var. saitoi | unsaturated oligogalacturonides | - |
? | |
apple pectin | - |
Aspergillus luchuensis var. saitoi KBN 2022 | unsaturated oligogalacturonides | - |
? | |
citrus peel pectin | - |
Aspergillus luchuensis var. saitoi | unsaturated oligogalacturonides | - |
? | |
citrus peel pectin | - |
Aspergillus luchuensis var. saitoi KBN 2022 | unsaturated oligogalacturonides | - |
? | |
polygalacturonate | - |
Aspergillus luchuensis var. saitoi | unsaturated trigalacturonic acid + unsaturated tetragalacturonic acid | - |
? | |
polygalacturonate | - |
Aspergillus luchuensis var. saitoi KBN 2022 | unsaturated trigalacturonic acid + unsaturated tetragalacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Aspergillus luchuensis var. saitoi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | - |
Aspergillus luchuensis var. saitoi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, 71% residual activity | Aspergillus luchuensis var. saitoi |
70 | - |
1 h, 61% residual activity | Aspergillus luchuensis var. saitoi |
80 | - |
1 h, 46% residual activity | Aspergillus luchuensis var. saitoi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
539 | - |
Polygalacturonate | mutant V197D, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
598 | - |
apple pectin | mutant V197D, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
601 | - |
Polygalacturonate | wild-type, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
624 | - |
Polygalacturonate | mutant I140V, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
642 | - |
apple pectin | wild-type, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
688 | - |
citrus peel pectin | mutant V197D, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
694 | - |
citrus peel pectin | wild-type, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
703 | - |
apple pectin | mutant I140V, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
712 | - |
citrus peel pectin | mutant I140V, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aspergillus luchuensis var. saitoi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
citrus peel pectin | kcat/Km value of wild-type 715 ml/mg/s, of mutant I140V 765 ml/mg/s, of mutant V197D 756 ml/mg/s, respectively, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
additional information | - |
apple pectin | kcat/Km value of wild-type, 573 ml/mg/s, of mutant I140V 724 ml/mg/s, of mutant V197D 598 ml/mg/s, respectively, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi | |
additional information | - |
Polygalacturonate | kcat/Km value of wild-type, 812 ml/mg/s, of mutant I140V 583 ml/mg/s, of mutant V197D 579 ml/mg/s, respectively, pH 8.0, 30°C | Aspergillus luchuensis var. saitoi |