Literature summary for 4.2.2.2 extracted from

Zhou, Z.; Liu, Y.; Chang, Z.; Wang, H.; Leier, A.; Marquez-Lago, T.T.; Ma, Y.; Li, J.; Song, J.
Structure-based engineering of a pectate lyase with improved specific activity for ramie degumming (2017), Appl. Microbiol. Biotechnol., 101, 2919-2929 .

Search for more literature for 4.2.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
-	Paenibacillus sp. 0602

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of PelN, to 1.45 A resolution	Paenibacillus sp. 0602

Protein Variants

Protein Variants	Comment	Organism
D174A	mutation in Ca2+ binding site, complete loss of activity	Paenibacillus sp. 0602
K93I	1.75fold increase in specific activity, folding free energy decreases by 1.03 kcal/ml	Paenibacillus sp. 0602

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	residues D152, D174, and D178 form the Ca2+ ion binding sites	Paenibacillus sp. 0602

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus sp. 0602	W8CR80	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1602	-	wild-type, pH 9.8, 50°C	Paenibacillus sp. 0602
2797	-	mutant K93I, pH 9.8, 50°C	Paenibacillus sp. 0602

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonate	-	Paenibacillus sp. 0602	?	-	?
ramie fiber	-	Paenibacillus sp. 0602	?	-	?

Synonyms

Synonyms	Comment	Organism
PelN	-	Paenibacillus sp. 0602

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
67.5	-	wild-type	Paenibacillus sp. 0602

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Release 2023.1 (January 2023)