Protein Variants | Comment | Organism |
---|---|---|
E124I | mutant displays increased thermostability | Bacillus sp. N16-5 |
E124I/T178A/N186D/I211V/A254T/S271G/N289H | 140fold increase in the t50 value at 50°C, accompanied by an 84.3% decrease in activity | Bacillus sp. N16-5 |
K313R | mutation does not improve thermostability | Bacillus sp. N16-5 |
N186D/I211V/A254T/S271G/N289H | 24fold increase in the t50 value at 50°C, with a 23.3% increase in activity | Bacillus sp. N16-5 |
R72S | mutation does not improve thermostability | Bacillus sp. N16-5 |
S233C | mutation does not improve thermostability | Bacillus sp. N16-5 |
S271G | mutant displays increased thermostability | Bacillus sp. N16-5 |
S308L | mutation does not improve thermostability | Bacillus sp. N16-5 |
T178A | mutant displays increased thermostability | Bacillus sp. N16-5 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
polygalacturonic acid | Km value is 1.13 mg/ml for wild-type, 1.20 mg/ml for mutant S271G, 1.12 mg/ml for mutant E241I, 1.28 mg/ml for mutant T178A, 1.74 mg/ml for mutant N186D/I211V/A254T/S271G/N289H, and 3.96 mg/ml for mutant 124I/T178A/N186D/I211V/A254T/S271G/N289H, respectively, pH 10.5, 50°C | Bacillus sp. N16-5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. N16-5 | D0VP31 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
293 | - |
mutant 124I/T178A/N186D/I211V/A254T/S271G/N289H, pH 10.5, 50°C | Bacillus sp. N16-5 |
1870 | - |
wild-type, pH 10.5, 50°C | Bacillus sp. N16-5 |
2305 | - |
mutant N186D/I211V/A254T/S271G/N289H, pH 10.5, 50°C | Bacillus sp. N16-5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid | - |
Bacillus sp. N16-5 | unsaturated oligogalacturonides | - |
? | |
ramie fiber | - |
Bacillus sp. N16-5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PelA | - |
Bacillus sp. N16-5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
time at which the enzyme loses 50% of its initial activity, i.e. t50 value, is 10 min for wild-type, 240 min for mutant N186D/I211V/A254T/S271G/N289H, 1400 min for mutant E124I/T178A/N186D/I211V/A254T/S271G/N289H, respectively | Bacillus sp. N16-5 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
526 | - |
polygalacturonic acid | mutant 124I/T178A/N186D/I211V/A254T/S271G/N289H, pH 10.5, 50°C | Bacillus sp. N16-5 | |
1532 | - |
polygalacturonic acid | mutant T178A, pH 10.5, 50°C | Bacillus sp. N16-5 | |
2492 | - |
polygalacturonic acid | mutant E241I, pH 10.5, 50°C | Bacillus sp. N16-5 | |
3319 | - |
polygalacturonic acid | wild-type, pH 10.5, 50°C | Bacillus sp. N16-5 | |
3637 | - |
polygalacturonic acid | mutant S271G, pH 10.5, 50°C | Bacillus sp. N16-5 | |
3932 | - |
polygalacturonic acid | mutant N186D/I211V/A254T/S271G/N289H, pH 10.5, 50°C | Bacillus sp. N16-5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
polygalacturonic acid | kcat/Km value is 2932 ml/mg/s for wild-type, 3044 ml/mg/s for mutant S271G, 2219 ml/mg/s for mutant E241I, 1196 ml/mg/s for mutant T178A, 2255 ml/mg/s for mutant N186D/I211V/A254T/S271G/N289H, and 133 ml/mg/s for mutant 124I/T178A/N186D/I211V/A254T/S271G/N289H, respectively, pH 10.5, 50°C | Bacillus sp. N16-5 |