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Literature summary for 4.2.2.2 extracted from

  • Liu, Y.; Chen, G.; Wang, J.; Hao, Y.; Li, M.; Li, Y.; Hu, B.; Lu, F.
    Efficient expression of an alkaline pectate lyase gene from Bacillus subtilis and the characterization of the recombinant protein (2012), Biotechnol. Lett., 34, 109-115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Bsu11286Pel, DNA and amino acid sequence determination and analysis, recombinant expression in Bacillus subtilis strain WB600 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38600
-
x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE Bacillus subtilis
45498
-
x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
isolated from soil of a saline-alkaline field in Tianjin, China, gene Bsu11286Pel
-
Bacillus subtilis TCCC11286
-
isolated from soil of a saline-alkaline field in Tianjin, China, gene Bsu11286Pel
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 17.3fold from strain WB600 culture supernatant by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
444.6
-
purified recombinant enzyme, pH 9.0, 50°C Bacillus subtilis

Subunits

Subunits Comment Organism
? x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
alkaline pectate lyase
-
Bacillus subtilis
Apel
-
Bacillus subtilis
pectate transeliminase
-
Bacillus subtilis
Pel
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
purified recombinant enzyme, pH 9.0, 2 h, over 90% activity remaining Bacillus subtilis
40
-
purified recombinant enzyme, pH 9.0, 2 h, over 80% activity remaining Bacillus subtilis
50
-
purified recombinant enzyme, 2 h, 90% activity remaining Bacillus subtilis
60
-
purified recombinant enzyme, pH 9.0, half-life is 20 min, inactivation after 80 min Bacillus subtilis
70
-
purified recombinant enzyme, pH 9.0, half-life is about 12 min, inactivation after 60 min Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7 11 activity range Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11.5 purified recombinant enzyme, 2 h, stable up to pH 9.0, inactivation at pH 11.5 Bacillus subtilis

General Information

General Information Comment Organism
evolution the mature Apel is structurally related to the enzymes in the polysaccharide lyase family 1 Bacillus subtilis
additional information Pel structure modeling, overview Bacillus subtilis