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Literature summary for 4.2.2.2 extracted from

  • Mukhopadhyay, A.; Dasgupta, A.K.; Chattopadhyay, D.; Chakrabarti, K.
    Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles (2012), Biores. Technol., 116, 348-354.
    View publication on PubMed
Search for more literature for 4.2.2.2

Activating Compound

Activating Compound Comment Organism Structure
additional information activity of pectate lyase is 9fold higher at 90°C in the presence of hydroxyapatite nanoparticles than in the presence of 1 mM CaCl2 Priestia megaterium

Protein Variants

Protein Variants Comment Organism
additional information improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme kinetics and thermodynamics at 75°C and 90°C in presence or absence of hydroxyapatite nanoparticles, detailed overview Priestia megaterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, optimal at 1 mM Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium AK2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 18.6fold from strain AK2 culture supernatant by cation exchange chromatography and gel filtration Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
852
-
 purified native enzyme, pH 8.5, 55°C Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles Priestia megaterium
90
-
 half-life of pectate lyase is 60fold higher in the presence of hydroxyapatite nanoparticles than in the presence of 1 mM CaCl2. Thermodynamic analysis of the nanoparticle-induced stability reveals an enhanced entropy-enthalpy compensation by hydroxyapatite nanoparticles since a reciprocal linearity of the enthalpy-entropy change to 90°C is observed Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Priestia megaterium