Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Xanthomonas campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | at 1 mM | Xanthomonas campestris | |
Cu2+ | at 1 mM | Xanthomonas campestris | |
EDTA | at 1 mM | Xanthomonas campestris | |
Ni2+ | at 1 mM | Xanthomonas campestris | |
SDS | at 1 mM | Xanthomonas campestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, best at 0.05 mM | Xanthomonas campestris | |
Co2+ | 1.4fold at 1 mM | Xanthomonas campestris | |
Mn2+ | 1.7fold at 1 mM | Xanthomonas campestris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, recombinant His-tagged enzyme, SDS-PAGE | Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | - |
- |
- |
Xanthomonas campestris ACCC 10048 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography to homogeneity | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin P9311 | - |
Xanthomonas campestris | ? | - |
? | |
citrus pectin P9311 | - |
Xanthomonas campestris ACCC 10048 | ? | - |
? | |
citrus pectin P9436 | - |
Xanthomonas campestris | ? | - |
? | |
citrus pectin P9436 | - |
Xanthomonas campestris ACCC 10048 | ? | - |
? | |
citrus pectin P9561 | - |
Xanthomonas campestris | ? | - |
? | |
citrus pectin P9561 | - |
Xanthomonas campestris ACCC 10048 | ? | - |
? | |
additional information | the enzyme activity against polygalacturonic acid as 100%, r-PL D exhibits 91.7%, 47.3%, and 6.5% of the activity when pectin is methyl-esterified 34%, 70%, and 85%, respectively | Xanthomonas campestris | ? | - |
? | |
additional information | the enzyme activity against polygalacturonic acid as 100%, r-PL D exhibits 91.7%, 47.3%, and 6.5% of the activity when pectin is methyl-esterified 34%, 70%, and 85%, respectively | Xanthomonas campestris ACCC 10048 | ? | - |
? | |
polygalacturonic acid | - |
Xanthomonas campestris | ? | - |
? | |
polygalacturonic acid | - |
Xanthomonas campestris ACCC 10048 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant His-tagged enzyme, SDS-PAGE | Xanthomonas campestris |
Synonyms | Comment | Organism |
---|---|---|
alkaline pectate lyase | - |
Xanthomonas campestris |
PL D | - |
Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant His-tagged enzyme | Xanthomonas campestris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
30 | a low-temperature-active alkaline pectate lyase, 35% of maximal activity at 0°C, maximal activity at 30°C | Xanthomonas campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant His-tagged enzyme | Xanthomonas campestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | over 45% of maximal activity within this range | Xanthomonas campestris |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to family PF09492 | Xanthomonas campestris |
additional information | the enzyme from Xanthomonas campestris ACCC 10048 is a low-temperature-active alkaline pectate lyase | Xanthomonas campestris |