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Literature summary for 4.2.2.2 extracted from

  • Yuan, P.; Meng, K.; Wang, Y.; Luo, H.; Shi, P.; Huang, H.; Tu, T.; Yang, P.; Yao, B.
    A low-temperature-active alkaline pectate lyase from Xanthomonas campestris ACCC 10048 with high activity over a wide pH range (2012), Appl. Biochem. Biotechnol., 168, 1489-1500.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Xanthomonas campestris

Inhibitors

Inhibitors Comment Organism Structure
Cr3+ at 1 mM Xanthomonas campestris
Cu2+ at 1 mM Xanthomonas campestris
EDTA at 1 mM Xanthomonas campestris
Ni2+ at 1 mM Xanthomonas campestris
SDS at 1 mM Xanthomonas campestris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, best at 0.05 mM Xanthomonas campestris
Co2+ 1.4fold at 1 mM Xanthomonas campestris
Mn2+ 1.7fold at 1 mM Xanthomonas campestris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, recombinant His-tagged enzyme, SDS-PAGE Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
-
-
-
Xanthomonas campestris ACCC 10048
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography to homogeneity Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin P9311
-
Xanthomonas campestris ?
-
?
citrus pectin P9311
-
Xanthomonas campestris ACCC 10048 ?
-
?
citrus pectin P9436
-
Xanthomonas campestris ?
-
?
citrus pectin P9436
-
Xanthomonas campestris ACCC 10048 ?
-
?
citrus pectin P9561
-
Xanthomonas campestris ?
-
?
citrus pectin P9561
-
Xanthomonas campestris ACCC 10048 ?
-
?
additional information the enzyme activity against polygalacturonic acid as 100%, r-PL D exhibits 91.7%, 47.3%, and 6.5% of the activity when pectin is methyl-esterified 34%, 70%, and 85%, respectively Xanthomonas campestris ?
-
?
additional information the enzyme activity against polygalacturonic acid as 100%, r-PL D exhibits 91.7%, 47.3%, and 6.5% of the activity when pectin is methyl-esterified 34%, 70%, and 85%, respectively Xanthomonas campestris ACCC 10048 ?
-
?
polygalacturonic acid
-
Xanthomonas campestris ?
-
?
polygalacturonic acid
-
Xanthomonas campestris ACCC 10048 ?
-
?

Subunits

Subunits Comment Organism
? x * 38000, recombinant His-tagged enzyme, SDS-PAGE Xanthomonas campestris

Synonyms

Synonyms Comment Organism
alkaline pectate lyase
-
Xanthomonas campestris
PL D
-
Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant His-tagged enzyme Xanthomonas campestris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
30 a low-temperature-active alkaline pectate lyase, 35% of maximal activity at 0°C, maximal activity at 30°C Xanthomonas campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant His-tagged enzyme Xanthomonas campestris

pH Range

pH Minimum pH Maximum Comment Organism
3 12 over 45% of maximal activity within this range Xanthomonas campestris

General Information

General Information Comment Organism
evolution the enzyme belongs to family PF09492 Xanthomonas campestris
additional information the enzyme from Xanthomonas campestris ACCC 10048 is a low-temperature-active alkaline pectate lyase Xanthomonas campestris