Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Caldicellulosiruptor bescii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant N-terminally His6-tagged enzyme, sitting drop vapor diffusion method, mixing of 0.0002 ml of protein containing 15 mg/ml protein, 20 mM acetic acid, pH 5, 100 mM NaCl and 5 mM CaCl2, with 0.0002 ml of well solution, containing 0.2 M ammonium phosphate monobasic, 0.1 M Tris, pH 8.5, and 50% v/v 2-methyl-2,4-pentanediol, 20°C, X-ray diffraction structure determination and analysis at 1.5 A resolution, molecular replacement method, modeling | Caldicellulosiruptor bescii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor bescii | B9MKT4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration | Caldicellulosiruptor bescii |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to pectate lyase family 3 | Caldicellulosiruptor bescii |