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Literature summary for 4.2.2.2 extracted from

  • Damak, N.; Hadj-Taieb, N.; Bonnin, E.; Ben Bacha, A.; Gargouri, A.
    Purification and biochemical characterization of a novel thermoactive fungal pectate lyase from Penicillium occitanis (2011), Process Biochem., 46, 888-893.
No PubMed abstract available
Search for more literature for 4.2.2.2

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 2 mM Penicillium sp. 'occitanis'

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0136
-
 polygalacturonic acid pH 9.0, 60°C Penicillium sp. 'occitanis'
0.0199
-
 polygalacturonic acid pH 7.0, 60°C Penicillium sp. 'occitanis'

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium sp. 'occitanis'
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizes, best activating metal ion, optimally at 2 mM Penicillium sp. 'occitanis'
additional information divalent cations are required for maximum activity Penicillium sp. 'occitanis'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 39000, Pel1, SDS-PAGE Penicillium sp. 'occitanis'

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polygalacturonic acid Penicillium sp. 'occitanis'
-
 ?
-
 ?
polygalacturonic acid Penicillium sp. 'occitanis' CT1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp. 'occitanis'
-
-
-
Penicillium sp. 'occitanis' CT1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Pel1 is 7.7% glycosylated Penicillium sp. 'occitanis'

Purification (Commentary)

Purification (Comment) Organism
native extracellular Pel1 71fold by anion exchange chromatography Penicillium sp. 'occitanis'

Source Tissue

Source Tissue Comment Organism Textmining
additional information highest level of Pel1 activity, 434 U/ml, is obtained in a medium supplemented with apple pectin Penicillium sp. 'occitanis'
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
194.3
-
 purified enzyme, pH 9.0, 60°C Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonic acid
-
 Penicillium sp. 'occitanis' ?
-
 ?
polygalacturonic acid
-
 Penicillium sp. 'occitanis' CT1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, Pel1, SDS-PAGE Penicillium sp. 'occitanis'

Synonyms

Synonyms Comment Organism
Pel1
-
 Penicillium sp. 'occitanis'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Penicillium sp. 'occitanis'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified native Pel1, half-life is 14.2 min at pH 7.0, 16.4 min at pH 9.0, the half-life is increased by Ca2+ Penicillium sp. 'occitanis'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Penicillium sp. 'occitanis'

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium sp. 'occitanis' Pel1, isoelectric focusing
-
 6.5