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Literature summary for 4.2.2.2 extracted from
- Arg235 is an essential catalytic residue of Bacillus pumilus DKS1 pectate lyase to degum ramie fibre (2011), Biodegradation, 22, 153-161.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the thermoactive pectate lyase from strain DKS1 of Bacillus pumilus has a potential use for degumming ramie fiber, the purified pectate lyase causes a weight loss of the ramie fibre of 25%. Arg235 is an essential catalytic residue to degumming ramie fibre | Bacillus pumilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pelB, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) plysS | Bacillus pumilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R235A | site-directed mutagenesis, inactive catalytic residue mutant | Bacillus pumilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Bacillus pumilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37556 | - |
x * 37556, sequence calculation | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | B2ZFM0 | gene pelB | - |
| Bacillus pumilus DKS1 | B2ZFM0 | gene pelB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) plysS 6.64fold by single step cation exchange chromatography to homogeneity | Bacillus pumilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
93 U/ml and weight of the ramie fibre of 1 g, pH 8.5, 37°C | Bacillus pumilus |
| 2893 | - |
purified recombinant wild-type enzyme, pH 8.5, 60°C | Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | Arg235 is an essential catalytic residue | Bacillus pumilus | ? | - |
? | |
| polygalacturonic acid | Arg235 is an essential catalytic residue | Bacillus pumilus DKS1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37556, sequence calculation | Bacillus pumilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DKS1 pectate lyase | - |
Bacillus pumilus |
| More | the enzyme belongs to the polysaccharide family 1 | Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus pumilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 100 | activity range | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus pumilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | - |
Bacillus pumilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus pumilus | sequence calculation | - |
9.23 |
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Release 2023.1 (January 2023)
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