Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and DH5alpha cells | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 25% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Ca2+ | Ca2+ does not increase but instead inhibits the activity of PelA, 41% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Co2+ | 22% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Cu2+ | 24% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
EDTA | PelA is sensitive to 10 mM EDTA with 39% activity retained | Bacillus sp. (in: Bacteria) | |
Hg2+ | 24% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Mg2+ | 56% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Mn2+ | complete inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
Ni2+ | 67% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Pb2+ | 53% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
SDS | PelA is sensitive to 0.1% (w/v) SDS with 43% activity retained | Bacillus sp. (in: Bacteria) | |
Sn2+ | 75% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Zn2+ | 69% residual activity at 1 mM | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ is not required for activity on pectic substrates | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35940 | - |
calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
42000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
45000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | D0VP31 | - |
- |
Bacillus sp. (in: Bacteria) N16-5 | D0VP31 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1000 | - |
after 25.3fold purification | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin | PelA shows 25% relative activity on citrus pectin compared to polygalacturonate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
citrus pectin | PelA shows 25% relative activity on citrus pectin compared to polygalacturonate | Bacillus sp. (in: Bacteria) N16-5 | ? | - |
? | |
additional information | PelA does not show any activity on mannan, CMC, xylan, glucan, or soluble starch | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | PelA does not show any activity on mannan, CMC, xylan, glucan, or soluble starch | Bacillus sp. (in: Bacteria) N16-5 | ? | - |
? | |
polygalacturonate | the enzyme is specific toward alpha-1,4-galacturonic acid linkages of galactopolysaccharides | Bacillus sp. (in: Bacteria) | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate + ? | main products | ? | |
polygalacturonate | the enzyme is specific toward alpha-1,4-galacturonic acid linkages of galactopolysaccharides | Bacillus sp. (in: Bacteria) N16-5 | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate + ? | main products | ? |
Synonyms | Comment | Organism |
---|---|---|
PelA | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | more than 60% of the maximum activity is detectable between 40 and 65°C | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | PelA is stable during 30°C to 40°C and not very stable at 50°C (in 50 mM glycine-NaOH buffer, pH 10.0), about 60% activity is retained after 30 min incubation at 50°C and it almost completely loses activity when it is incubated at 60°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | 12 | PelA is stable at pH 9.0 and 10.0 and maintains more than 85% activity after incubation at 40°C for 1 h, about 50% activity is retained when it is incubated at pH 11.0 and it almost completely loses activity when it is incubated at pH 12.0 | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | pI-value around pH 6.0, isoelectric focusing | - |
6 |