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Literature summary for 4.2.2.2 extracted from
- Purification, characterization, and overexpression of thermophilic pectate lyase of Bacillus sp. RN1 isolated from a hot spring in Thailand (2009), Biosci. Biotechnol. Biochem., 73, 268-273.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tris-HCl buffer | enzyme activity increases with increasing concentration of the buffer (20 mM to 1 M) and has highest activity at 1 M | Bacillus sp. (in: Bacteria) |  |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | useful for degradation of pectin networks at high temperatures | Bacillus sp. (in: Bacteria) |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | the enzyme is inhibited 30% in the presence of Co2+ | Bacillus sp. (in: Bacteria) | |
| EDTA | 5 mM EDTA causes complete loss of enzyme activity | Bacillus sp. (in: Bacteria) | |
| additional information | no effect on activity is detected with KCl or NaCl | Bacillus sp. (in: Bacteria) | |
| Ni2+ | the enzyme is inhibited 30% in the presence of Ni2+ | Bacillus sp. (in: Bacteria) | |
| Zn2+ | the enzyme is inhibited 30% in the presence of Zn2+ | Bacillus sp. (in: Bacteria) | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | the enzyme is activated slightly in the presence of BaCl2 | Bacillus sp. (in: Bacteria) | |
| Ca2+ | required for activity | Bacillus sp. (in: Bacteria) | |
| Mg2+ | the enzyme is activated 40% in the presence of MgCl2 | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 33450 | - |
calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | B1B6T1 | - |
- |
| Bacillus sp. (in: Bacteria) RN1 | B1B6T1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM-Toyopearl 650M column chromatography, Mono S column chromatography, Resource PHE column chromatography, and ammonium sulfate precipitation, recombinant enzyme is purified by amylase resin column chromatography | Bacillus sp. (in: Bacteria) |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.065 | - |
culture filtrate, in 20 mM Tris-HCl pH 8.0, at 60°C | Bacillus sp. (in: Bacteria) |
| 12 | - |
after 185fold purification, in 20 mM Tris-HCl pH 8.0, at 60°C | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | - |
Bacillus sp. (in: Bacteria) | 4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid | unsaturated di- and trigalacturonic acids are mainly formed as the final products of degradation by Pel SWU | ? | |
| polygalacturonic acid | - |
Bacillus sp. (in: Bacteria) RN1 | 4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid | unsaturated di- and trigalacturonic acids are mainly formed as the final products of degradation by Pel SWU | ? | |
| polygalacturonic acid | specific substrate, enzyme activity decreases when the methoxyl content of the substrate increases | Bacillus sp. (in: Bacteria) | unsaturated oligogalacturonate + ? | - |
? | |
| polygalacturonic acid | specific substrate, enzyme activity decreases when the methoxyl content of the substrate increases | Bacillus sp. (in: Bacteria) RN1 | unsaturated oligogalacturonate + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pel SWU | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
at pH 8.0 | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | the enzyme loses 60% of its activity when incubated at 40°C for 1 h after treatment with EDTA, thermostability increases to approximately 80% of initial activity at 50 and 70°C in the presence of Ca2+ alone and both Ca2+ and polygalacturonic acid, respectively | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | at 4°C | Bacillus sp. (in: Bacteria) |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | calculated from amino acid sequence | - |
9.1 |
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