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Literature summary for 4.2.2.2 extracted from
- Efficient secretory expression of an alkaline pectate lyase gene from Bacillus subtilis in E. coli and the purification and characterization of the protein (2007), Biotechnol. Lett., 29, 405-410.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene encoding pectate lyase is amplified by PCR, fused with a periplasmic secretion signal peptide sequence, pelB, from pET22b(+), cloned and expressed in Escherichia coli cells using a temperature control vector, pHsh | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis WSHB04-02 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate | the enzyme produces unsaturated oligo-galacturonides including unsaturated tri-galacturonic acid and unsaturated bi-galacturonic acid but not unsaturated mono-galacturonic acid | Bacillus subtilis | unsaturated oligo-galacturonides | - |
? |  |
| polygalacturonate | the enzyme produces unsaturated oligo-galacturonides including unsaturated tri-galacturonic acid and unsaturated bi-galacturonic acid but not unsaturated mono-galacturonic acid | Bacillus subtilis WSHB04-02 | unsaturated oligo-galacturonides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline pectate lyase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 40°C: about 30% of maximal activity, 45°C: about 55% of maximal activity, 70°C: about 35% of maximal activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 9.4: stable for 300 h. pH 10.0: about 40% loss of activity after 300 h | Bacillus subtilis |
| 50 | - |
stable for 30 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | 10 | - |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | 10.3 | pH 8.3: about 45% of maximal activity, pH 10.3: about 40% of maximal activity | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
45°C, stable for 300 h | Bacillus subtilis |
| 9.7 | - |
45°C, about 30% loss of activity after 300 h | Bacillus subtilis |
| 10.5 | - |
45°C, about 40% loss of activity after 300 h | Bacillus subtilis |
| 10.5 | - |
45°C, about 55% loss of activity after 300 h | Bacillus subtilis |
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