Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme can be used for bioscouring of cotton | Bacillus pumilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.38 mM, 50% inhibition | Bacillus pumilus | |
EDTA | 0.09 mM, 50% inhibition | Bacillus pumilus | |
Zn2+ | 2.8 mM, 50% inhibition | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value for polygalacturonate is 0.24 g/l, at pH 7.8, 50°C. KM-value is 0.24 g/l for not esterified pectin, 0.36 g/l for pectin with 5.3% esterification, 0.35 g/l for pectin with 29.5% esterification, 1.34 g/l for pectin with 51.4% esterification | Bacillus pumilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus pumilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme does not require Ca2+ | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37300 | - |
x * 37300, SDS-PAGE | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
- |
- |
Bacillus pumilus BK2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
esterified pectin | affinity shows a maximum for intermediate esterified pectins and decreases over a value of 50% of esterification. The best substrate is 29.5% methylated pectin | Bacillus pumilus | unsaturated tetragalacturonic acid | - |
? | |
esterified pectin | affinity shows a maximum for intermediate esterified pectins and decreases over a value of 50% of esterification. The best substrate is 29.5% methylated pectin | Bacillus pumilus BK2 | unsaturated tetragalacturonic acid | - |
? | |
polygalacturonate | the enzyme cleaves polygalacturonic acid via a beta-elimination mechanism | Bacillus pumilus | unsaturated tetragalacturonic acid | - |
? | |
polygalacturonate | the enzyme cleaves polygalacturonic acid via a beta-elimination mechanism | Bacillus pumilus BK2 | unsaturated tetragalacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37300, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life: more than 75 h | Bacillus pumilus |
80 | - |
half-life at pH 8.0 and 7.0 is 1.2 min, half-life at pH 9.0 is 0.8 min | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus pumilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus pumilus | 2D PAGE, pH-range 3-10 | - |
8.5 |