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Literature summary for 4.2.2.2 extracted from

  • Miyairi, K.; Ogasawara, A.; Tonouchi, A.; Hosaka, K.; Kudou, M.; Okuno, T.
    Low-molecular-weight pectate lyase from Streptomyces thermocarboxydus (2004), J. Appl. Glycosci., 51, 1-7.
No PubMed abstract available
Search for more literature for 4.2.2.2

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximal activity at 0.6 mM Streptomyces thermocarboxydus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Streptomyces thermocarboxydus

Organism

Organism UniProt Comment Textmining
Streptomyces thermocarboxydus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pectate lyase I Streptomyces thermocarboxydus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oligogalacturonic acid the enzyme acts on polygalacturonic acids and oligogalacturonic acids over digalacturonic acid and better on the larger galacturonic acids until at least DP8 Streptomyces thermocarboxydus ?
-
 ?
pectin better substrate for pectate lyase I than polygalacturonic acid Streptomyces thermocarboxydus ?
-
 ?
polygalacturonic acid endo-type reaction, little action on highly esterified polygalacturonic acid methylglucoside.The enzyme acts on polygalacturonic acids and oligogalacturonic acids over digalacturonic acid and better on the larger galacturonic acids until at least DP8 Streptomyces thermocarboxydus 4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Streptomyces thermocarboxydus

Synonyms

Synonyms Comment Organism
pectate lyase I
-
 Streptomyces thermocarboxydus
PL I
-
 Streptomyces thermocarboxydus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Streptomyces thermocarboxydus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Streptomyces thermocarboxydus