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Literature summary for 4.2.2.2 extracted from

  • Ogawa, A.; Sawada, K.; Saito, K.; Hakamada, Y.; Sumitomo, N.; Hatada, Y.; Kobayashi, T.; Ito, S.
    A new high-alkaline and high-molecular-weight pectate lyase from a Bacillus isolate: enzymatic properties and cloning of the gene for the enzyme (2000), Biosci. Biotechnol. Biochem., 64, 1133-1141.
    View publication on PubMed
Search for more literature for 4.2.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, in 50 mM Tris-HCl buffer, pH 7.5, 10 min at 30°C, complete inhibition, activity recovered by addition of 0.1 mM CaCl2 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 gel filtration Bacillus sp. (in: Bacteria)
70000
-
 1 * 70000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q9XDT1
-
-
Bacillus sp. (in: Bacteria) KSM-P15 Q9XDT1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin with degrees of esterification of 31%, 63% and 94% is degraded with 103%, 84% and 46% of the activity with polygalacturonic acid Bacillus sp. (in: Bacteria) ?
-
 ?
pectin with degrees of esterification of 31%, 63% and 94% is degraded with 103%, 84% and 46% of the activity with polygalacturonic acid Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?
polygalacturonic acid degradation through an endo-type fashion Bacillus sp. (in: Bacteria) ?
-
 ?
polygalacturonic acid degradation through an endo-type fashion Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
pectate transeliminase
-
 Bacillus sp. (in: Bacteria)
Pel
-
 Bacillus sp. (in: Bacteria)
Pel-15H
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 35% of maximal activity, 70°C: about 20% of maximal activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
9.5 12 pH 9.5: about 50% of maximal activity, pH 12.0: about 60% of maximal activity Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing, pH-gradient: 3.5-9.5 4.7 4.6