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Literature summary for 4.2.2.2 extracted from

  • Brühlmann, F.
    Purification and characterization of an extracellular pectate lyase from an amycolata sp. (1995), Appl. Environ. Microbiol., 61, 3580-3585.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.2

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits activity with polygalacturonate, no inhibition of the ability to cleave protopectin of Boehmeria nivea Pseudonocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for polygalacturonate: 0.019 g/l Pseudonocardia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudonocardia sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Pseudonocardia sp.
Ca2+ maximal activity at 0.2 mM Ca2+ Pseudonocardia sp.
Mg2+ 41% of the activation with Ca2+ Pseudonocardia sp.
Sr2+ 21% of the activation with Ca2+ Pseudonocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 31000, SDS-PAGE Pseudonocardia sp.
10000000
-
 matrix-assisted laser desorption ionization mass spectrometry Pseudonocardia sp.

Organism

Organism UniProt Comment Textmining
Pseudonocardia sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification contains no carbohydrate Pseudonocardia sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudonocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin no activity with 93% esterified pectin Pseudonocardia sp. ?
-
 ?
polygalacturonic acid endo-cleavage Pseudonocardia sp. unsaturated oligogalacturonate wide range of 4,5-unsaturated oligogalacturonates, further depolymerized to unsaturated dimer and trimer ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Pseudonocardia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Pseudonocardia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Ca2+ and sodium polygalacturonate improve thermal stability Pseudonocardia sp.
50
-
 1 h, loss of activity Pseudonocardia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 optimal stability in the range Pseudonocardia sp.