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Literature summary for 4.2.2.2 extracted from
- Purification, characterization, and production of two pectic transeliminases with protopectinase activity from Bacillus subtilis (1994), Biosci. Biotechnol. Biochem., 58, 353-358.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Bacillus subtilis |  |
| Ba2+ | Ba(CH3CO2)2 | Bacillus subtilis | |
| CdCl2 | - |
Bacillus subtilis | |
| Co2+ | CoCl2 | Bacillus subtilis | |
| Cu2+ | CuSO4 | Bacillus subtilis | |
| Fe2+ | FeCl2 | Bacillus subtilis | |
| Hg2+ | HgCl2 | Bacillus subtilis | |
| Mn2+ | MnCl2 | Bacillus subtilis | |
| Ni2+ | NiCl2 | Bacillus subtilis | |
| Zn2+ | ZnCl2 | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Bacillus subtilis |
| 43000 | - |
x * 43000, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus subtilis | enzyme production is repressed by glucose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
IFO 3134 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Bacillus subtilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme production is repressed by glucose | Bacillus subtilis | ? | - |
? | |
| pectin | - |
Bacillus subtilis | ? | - |
? | |
| polygalacturonic acid | activity decreases when the methoxyl content of the substrate increases | Bacillus subtilis | unsaturated oligogalacturonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
at pH 6.0 | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | pH 6.0, 30 min, stable | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
at 37°C | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | 37°C, 16 h, stable | Bacillus subtilis |
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