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Literature summary for 4.2.2.2 extracted from

  • McCarthy, R.E.; Kotarsky, S.F.; Salyers, A.A.
    Location and characteristics of enzymes involved in the breakdown of polygalacturonic acid by Bacteroides thetaiotaomicron (1985), J. Bacteriol., 161, 493-499.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for poly-D-galacturonic acid: 0.040-0.070 mg/ml Bacteroides thetaiotaomicron

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with membranes under certain conditions Bacteroides thetaiotaomicron 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bacteroides thetaiotaomicron

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, SDS-PAGE Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacteroides thetaiotaomicron

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
 Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonic acid
-
 Bacteroides thetaiotaomicron unsaturated oligogalacturonate the main product appears to be a disaccharide that contains a DELTA4,5-unsaturated galacturonic acid residue ?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE Bacteroides thetaiotaomicron

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Bacteroides thetaiotaomicron