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Literature summary for 4.2.2.19 extracted from

  • Wu, J.; Ji, Y.; Su, N.; Li, Y.; Liu, X.; Mei, X.; Zhou, Q.; Zhang, C.; Xing, X.H.
    Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin (2016), Carbohydr. Polym., 144, 338-345 .
    View publication on PubMed

Application

Application Comment Organism
analysis method for quantitative detection of dermatan sulfate in heparin. Selective detection of deramtan sulfate in heparin is possible based on the increased value of UV absorption at 232 nm resulting from exhaustive depolymerization of dermatan sulfate. The first method facilitates analysis in heparin with dermatan sulfate concentrations greater than 0.1 mg/ml at 232 nm, with good linearity, precision and recovery. The second method allows sensitive and accurate detection of dermatan sulfate at concentrations below 0.1 mg/ml, with good linearity, precision and recovery. The linear range of dermatan sulfate detection is between 0.01 and 0.5 mg/ml Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus Q46079
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-
Pedobacter heparinus DSM 2366 Q46079
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dermatan sulfate
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Pedobacter heparinus oligosaccharides
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?
dermatan sulfate
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Pedobacter heparinus DSM 2366 oligosaccharides
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?

Synonyms

Synonyms Comment Organism
ChonB
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Pedobacter heparinus
CslB
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Pedobacter heparinus