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Literature summary for 4.2.2.19 extracted from

  • Pojasek, K.; Shriver, Z.; Kiley, P.; Venkataraman, G.; Sasisekharan, R.
    Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum (2001), Biochem. Biophys. Res. Commun., 286, 343-351.
    View publication on PubMed
Search for more literature for 4.2.2.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as N-terminally His-tagged enzyme, 2 different expression systems Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028 0.0046 dermatan sulfate recombinant enzyme, pH 8.0, 30°C, dependent on expression system Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54070
-
 mass spectrometry Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus Q46079
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli, His-tag is removed, purified to homogeneity, 69fold Pedobacter heparinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80.8
-
 purified recombinant enzyme Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dermatan sulfate
-
 Pedobacter heparinus ?
-
 ?

Synonyms

Synonyms Comment Organism
chondroitinase B
-
 Pedobacter heparinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pedobacter heparinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
87 190 dermatan sulfate recombinant enzyme, pH 8.0, 30°C, dependent on expression system Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pedobacter heparinus