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Literature summary for 4.2.2.18 extracted from

  • Li, Y.; Miao, M.; Chen, X.; Jiang, B.; Liu, M.; Feng, B.
    Improving the catalytic behavior of inulin fructotransferase under high hydrostatic pressure (2015), J. Sci. Food Agric., 95, 2588-2594 .
    View publication on PubMed
Search for more literature for 4.2.2.18

Application

Application Comment Organism
synthesis use of high hydrostatic pressure to improve the catalytic efficiency. The maximum activity of IFTase is obtained under 200 MPa at 60°C. High hydrostatic pressure lowers the energy barrier of the enzymatic reaction and decreases the volume between the reactants and the transition state. Under this condition, the optimal pH for the enzymatic reaction shifts from 5.5 to 6.0. The activity is further enhanced by 65.2% in the presence of 1.5 M NaCl Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.442
-
 inulin pH 5.5, 60°C, pressure of 200 MPa Paenarthrobacter aurescens
0.493
-
 inulin pH 5.5, 60°C, pressure of 150 MPa Paenarthrobacter aurescens
0.538
-
 inulin pH 5.5, 60°C, pressure of 100 MPa Paenarthrobacter aurescens
0.57
-
 inulin pH 5.5, 60°C, pressure of 0.1 MPa Paenarthrobacter aurescens

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ the activity is enhanced by 65.2% in the presence of 1.5 M NaCl Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens F8QV43
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin
-
 Paenarthrobacter aurescens di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1054
-
 inulin pH 5.5, 60°C, pressure of 0.1 MPa Paenarthrobacter aurescens
1244
-
 inulin pH 5.5, 60°C, pressure of 100 MPa Paenarthrobacter aurescens
1327
-
 inulin pH 5.5, 60°C, pressure of 150 MPa Paenarthrobacter aurescens
1437
-
 inulin pH 5.5, 60°C, pressure of 200 MPa Paenarthrobacter aurescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1860
-
 inulin pH 5.5, 60°C, pressure of 0.1 MPa Paenarthrobacter aurescens
2309
-
 inulin pH 5.5, 60°C, pressure of 100 MPa Paenarthrobacter aurescens
2691
-
 inulin pH 5.5, 60°C, pressure of 150 MPa Paenarthrobacter aurescens
3246
-
 inulin pH 5.5, 60°C, pressure of 200 MPa Paenarthrobacter aurescens