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Literature summary for 4.2.2.18 extracted from

  • Yu, S.; Wang, X.; Zhang, T.; Jiang, B.; Mu, W.
    Probing the role of two critical residues in inulin fructotransferase (DFA III-producing) thermostability from Arthrobacter sp. 161MFSha2.1 (2016), J. Agric. Food Chem., 64, 6188-6195 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arthrobacter sp. 161MFSha2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. Residues Ser309 and Ser333 may play key roles in the structural stability Arthrobacter sp. 161MFSha2.1

Protein Variants

Protein Variants Comment Organism
S309A 95.8% of wild-type activity, 17.9 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S309A/S333A 88.2% of wild-type activity, 25.9 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S309C 94.9% of wild-type activity, 16.8 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S309C/S333C 84.5% of wild-type activity, 23.7 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S309F 102.6% of wild-type activity, 15.8 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S309F/S333F 96.4% of wild-type activity, 19.5 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S309T 103.5% of wild-type activity, 2.9 degrees increase in melting temperature Arthrobacter sp. 161MFSha2.1
S309T/S333T 91.8% of wild-type activity, 5.1 degrees increase in melting temperature Arthrobacter sp. 161MFSha2.1
S333A 98.6% of wild-type activity, 18.8 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S333C 95.7% of wild-type activity, 15.7 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S333F 97.4% of wild-type activity, 13.3 degrees decrease in melting temperature Arthrobacter sp. 161MFSha2.1
S333T 92.4% of wild-type activity, 2.2 degrees increase in melting temperature Arthrobacter sp. 161MFSha2.1

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. 161MFSha2.1 WP_018778058
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Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Arthrobacter sp. 161MFSha2.1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
mutants S309A, S309C, S309F, S333A, S333C, S333F, S309A/S333A, S309C/S333C, S309F/S333F Arthrobacter sp. 161MFSha2.1
55
-
wild-types, mutant S309T, S333T, S309T/S333T Arthrobacter sp. 161MFSha2.1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
87
-
wild-type, melting temperature Arthrobacter sp. 161MFSha2.1