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Literature summary for 4.2.2.18 extracted from
- Inulin fructotransferase (DFAIII-producing) from Arthrobacter ureafaciens D13-3 (2010), Carbohydr. Polym., 82, 742-746.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
inulin | pH 5.5, 50°C | Paenarthrobacter ureafaciens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Paenarthrobacter ureafaciens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
1 * 40000, SDS-PAGE | Paenarthrobacter ureafaciens |
| 43000 | - |
gel filtration | Paenarthrobacter ureafaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter ureafaciens | - |
- |
- |
| Paenarthrobacter ureafaciens D13-3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenarthrobacter ureafaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 290 | - |
pH 5.5, 50°C | Paenarthrobacter ureafaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin | - |
Paenarthrobacter ureafaciens | di-D-fructofuranose 1,2':2,3' dianhydride + ? | minor products are sucrose, 1-kestose and nystose | ? | |
| inulin | - |
Paenarthrobacter ureafaciens D13-3 | di-D-fructofuranose 1,2':2,3' dianhydride + ? | minor products are sucrose, 1-kestose and nystose | ? | |
| nystose | smallest fructo-oligosaccharide accepted as substrate | Paenarthrobacter ureafaciens | di-D-fructofuranose 1,2':2,3' dianhydride + sucrose | - |
? | |
| nystose | smallest fructo-oligosaccharide accepted as substrate | Paenarthrobacter ureafaciens D13-3 | di-D-fructofuranose 1,2':2,3' dianhydride + sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Paenarthrobacter ureafaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Paenarthrobacter ureafaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 65 | more than 50% of maximum activity within | Paenarthrobacter ureafaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable up to 30 min | Paenarthrobacter ureafaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Paenarthrobacter ureafaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | more than 50% of maximum activity within | Paenarthrobacter ureafaciens |
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Release 2023.1 (January 2023)
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