Literature summary for 4.2.2.18 extracted from

Momma, M.; Fujimoto, Z.; Maita, N.; Haraguchi, K.; Mizuno, H.
Expression, crystallization and preliminary X-ray crystallographic studies of Arthrobacter globiformis inulin fructotransferase (2003), Acta Crystallogr. Sect. D, D59, 2286-2288.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Arthrobacter globiformis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are obtained at 20°C by hanging drop vapour-diffusion method using 0.1 M Na HEPES pH 7.5 buffer containing 1.5 M lithium sulfate as a precipitant. Crystals of the recombinant wild-type enzyme diffract to better than 1.5 A at -173°C. The crystals belong to space group R32, with unit-cell parameters a = b = 90.02 A, c = 229.82 A in the hexagonal axes. Selenomethionine-derivative crystals belong to a different space group, C2, with unit-cell parameters a = 159.32, cb = 91.92, c = 92.58 A, beta = 125.06. The C2 selenomethionine-derived crystal contains three molecules per asymmetric unit	Arthrobacter globiformis

Crystallization (Comment)

Organism

crystals are obtained at 20°C by hanging drop vapour-diffusion method using 0.1 M Na HEPES pH 7.5 buffer containing 1.5 M lithium sulfate as a precipitant. Crystals of the recombinant wild-type enzyme diffract to better than 1.5 A at -173°C. The crystals belong to space group R32, with unit-cell parameters a = b = 90.02 A, c = 229.82 A in the hexagonal axes. Selenomethionine-derivative crystals belong to a different space group, C2, with unit-cell parameters a = 159.32, cb = 91.92, c = 92.58 A, beta = 125.06. The C2 selenomethionine-derived crystal contains three molecules per asymmetric unit

Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	3 * 43000, SDS-PAGE	Arthrobacter globiformis

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter globiformis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Arthrobacter globiformis

Subunits

Subunits	Comment	Organism
trimer	3 * 43000, SDS-PAGE	Arthrobacter globiformis

Synonyms

Synonyms	Comment	Organism
IFTaseIII	-	Arthrobacter globiformis
inulin fructotransferase (DFAIII-producing)	-	Arthrobacter globiformis

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Release 2023.1 (January 2023)