KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
inulin | with MW of inulin taken as 5000 Da | Arthrobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Arthrobacter sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, SDS-PAGE | Arthrobacter sp. |
100000 | - |
gel filtration | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
gene ift | - |
Purification (Comment) | Organism |
---|---|
from strain H65-7 | Arthrobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Arthrobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
604 | - |
purified enzyme | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin | other substrates: sucrose, 1-kestose, nystose, 1-F-fructofuranosyl-nystose, raffinose, melibiose, melezitose, stachyose, bacterial levans | Arthrobacter sp. | 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? | after exhaustive digestion of inulin nystose and 1-F-fructofuranosyl-nystose are produced in addition to di-D-fructofuranose 1,2':2,3' dianhydride | ? | |
inulin | other substrates: sucrose, 1-kestose, nystose, 1-F-fructofuranosyl-nystose, raffinose, melibiose, melezitose, stachyose, bacterial levans | Arthrobacter sp. | 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? | + oligosaccharides, small amount | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, SDS-PAGE | Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Arthrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
about 50% of activity maximum at 40°C and 75°C | Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable up to | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
strain H65-7, pI: 4.7 | Arthrobacter sp. |
5.5 | - |
- |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | about 50% of activity maximum at pH 4.0 and pH 8.0 | Arthrobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
stable | Arthrobacter sp. |