Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.18 extracted from

  • Yokota, A.; Enomoto, K.; Tomita, F.
    Purification and properties of inulin fructotransferase (depolymerizing) from Arhrobacter sp. H65-7 (1991), J. Ferment. Bioeng., 72, 262-265.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
inulin with MW of inulin taken as 5000 Da Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Arthrobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, SDS-PAGE Arthrobacter sp.
100000
-
gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
gene ift
-

Purification (Commentary)

Purification (Comment) Organism
from strain H65-7 Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
604
-
purified enzyme Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin other substrates: sucrose, 1-kestose, nystose, 1-F-fructofuranosyl-nystose, raffinose, melibiose, melezitose, stachyose, bacterial levans Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? after exhaustive digestion of inulin nystose and 1-F-fructofuranosyl-nystose are produced in addition to di-D-fructofuranose 1,2':2,3' dianhydride ?
inulin other substrates: sucrose, 1-kestose, nystose, 1-F-fructofuranosyl-nystose, raffinose, melibiose, melezitose, stachyose, bacterial levans Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? + oligosaccharides, small amount ?

Subunits

Subunits Comment Organism
dimer 2 * 49000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
about 50% of activity maximum at 40°C and 75°C Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
stable up to Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
strain H65-7, pI: 4.7 Arthrobacter sp.
5.5
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8 about 50% of activity maximum at pH 4.0 and pH 8.0 Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
stable Arthrobacter sp.