Cloned (Comment) | Organism |
---|---|
NanB sequence lacking the first 29 residues is PCR-amplified from Streptococcus pneumoniae DNA, cloned into the pOPINF vector and expressed in Escherichia coli | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
crystal structure of NanB in complex with its reaction product 2,7-anhydro-Neu5Ac is reported. NanB crystal structure consists of an N-terminal lectin-like domain (L-domain, residues 30-227), a catalytic six-bladed beta-propeller domain (N-domain, residues 228-345 and 457-697), and an irregular beta-stranded domain inserted into the catalytic domain (I-domain, residues 346-456) | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human alpha-1 acid glycoprotein + H2O | NanB preferentially cleaves alpha2-3 linked sialic acid from human alpha-1 glycoprotein | Streptococcus pneumoniae | 2,7-anhydro-alpha-N-acetylneuraminic acid + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NanB | - |
Streptococcus pneumoniae |
neuraminidase B | - |
Streptococcus pneumoniae |