Application | Comment | Organism |
---|---|---|
biotechnology | a glucuronan lyase is immobilized on a monolithic Convective Interaction Media disk. Degradations of three glucuronans with various O-acetylation degrees is investigated and compared with degradations using free enzyme. The immobilized glucuronan lyase is inhibited by the O-acetylation degree like the free enzyme. 1H NMR analyses are used to study the O-acetylation degree of oligoglucuronans and demonstrate that the average degrees of polymerization are inclusive between 4 and 13 after 24 h of degradation. This first immobilization of a glucuronan lyase constitutes a tool to produce oligoglucuronans | Trichoderma sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration and anion-exchange chromatography | Trichoderma sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
immobilized glucuronan lyase activity is calculated for deacetylated glucuronan. The activity is 1300times less than the theoretical immobilized activity. Activity decreases 4times and 14times for native and highly acetylated glucuronans, respectively | Trichoderma sp. |
Synonyms | Comment | Organism |
---|---|---|
glucuronan lyase | - |
Trichoderma sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Trichoderma sp. |