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Literature summary for 4.2.2.11 extracted from

  • Manns, D.; Nyffenegger, C.; Saake, B.; Meyer, A.
    Impact of different alginate lyases on combined cellulase-lyase saccharification of brown seaweed (2016), RSC Adv., 6, 45392-45401 .
No PubMed abstract available
Search for more literature for 4.2.2.11

Application

Application Comment Organism
degradation enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata Sphingobacterium multivorum
degradation enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata Sphingomonas sp. A1
degradation enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata Flavobacterium sp. S20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingomonas sp. A1
expression in Escherichia coli Flavobacterium sp. S20

Organism

Organism UniProt Comment Textmining
Flavobacterium sp. S20 G9CHX6
-
-
Sphingobacterium multivorum
-
-
-
Sphingomonas sp. A1 Q75WP3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Sphingobacterium multivorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Sphingobacterium multivorum unsaturated algino-oligosaccharides
-
 ?
alginate
-
 Sphingomonas sp. A1 unsaturated algino-oligosaccharides
-
 ?
alginate
-
 Flavobacterium sp. S20 unsaturated algino-oligosaccharides
-
 ?
additional information enzyme acts only on poly-guluronate Sphingobacterium multivorum ?
-
 ?
additional information enzyme prefers polyG blocks over polyM blocks Sphingomonas sp. A1 ?
-
 ?
additional information enzyme prefers polyM blocks over polyG blocks Flavobacterium sp. S20 ?
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Sphingobacterium multivorum 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Sphingomonas sp. A1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Flavobacterium sp. S20 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3 Sphingomonas sp. A1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3 Flavobacterium sp. S20 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Synonyms

Synonyms Comment Organism
Alg2A
-
 Sphingobacterium multivorum
Alg2A
-
 Flavobacterium sp. S20

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Sphingomonas sp. A1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
 Sphingomonas sp. A1
7.5
-
-
 Sphingobacterium multivorum
7.5
-
-
 Flavobacterium sp. S20