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Literature summary for 4.2.2.11 extracted from

  • Pei, X.; Chang, Y.; Shen, J.
    Cloning, expression and characterization of an endo-acting bifunctional alginate lyase of marine bacterium Wenyingzhuangia fucanilytica (2019), Protein Expr. Purif., 154, 44-51 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Wenyingzhuangia fucanilytica

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 83% of initial activity Wenyingzhuangia fucanilytica
Hg2+ 1 mM, 34% of initial activity Wenyingzhuangia fucanilytica
Li+ 1 mM, 65% of initial activity Wenyingzhuangia fucanilytica
Mg2+ 1 mM, 65% of initial activity Wenyingzhuangia fucanilytica
Mn2+ 1 mM, 69% of initial activity Wenyingzhuangia fucanilytica
Ni2+ 1 mM, 32% of initial activity Wenyingzhuangia fucanilytica
SDS 1 mM, 29% of initial activity Wenyingzhuangia fucanilytica
Zn2+ 1 mM, 25% of initial activity Wenyingzhuangia fucanilytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0237
-
alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 113% of initial activity Wenyingzhuangia fucanilytica

Organism

Organism UniProt Comment Textmining
Wenyingzhuangia fucanilytica A0A1B1Y7V0 bifunctional alginate lyase, catalyzes both reactions of EC 4.2.2.3 and EC 4.2.2.11
-

Purification (Commentary)

Purification (Comment) Organism
-
Wenyingzhuangia fucanilytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.48
-
substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C Wenyingzhuangia fucanilytica
23.34
-
substrate alginate, pH 8.0, 35°C Wenyingzhuangia fucanilytica
27.03
-
substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C Wenyingzhuangia fucanilytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
Wenyingzhuangia fucanilytica unsaturated algino-oligosaccharides
-
?
poly(alpha-(1,4)-L-guluronate)
-
Wenyingzhuangia fucanilytica 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
?
poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3 Wenyingzhuangia fucanilytica 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
?

Subunits

Subunits Comment Organism
? x * 33900, calculated from sequence, x * 34000, SDS-PAGe recombinant protein with His-tag Wenyingzhuangia fucanilytica

Synonyms

Synonyms Comment Organism
AXE80_11190
-
Wenyingzhuangia fucanilytica
xadAly7B
-
Wenyingzhuangia fucanilytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Wenyingzhuangia fucanilytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
24 h, 86% residual activity Wenyingzhuangia fucanilytica
35
-
24 h, 76% residual activity Wenyingzhuangia fucanilytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.94
-
alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Wenyingzhuangia fucanilytica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.81
-
alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica