Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.11 extracted from

  • Zhu, B.; Tan, H.; Qin, Y.; Xu, Q.; Du, Y.; Yin, H.
    Characterization of a new endo-type alginate lyase from Vibrio sp. W13 (2015), Int. J. Biol. Macromol., 75, 330-337 .
    View publication on PubMed
Search for more literature for 4.2.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp. W13

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 80% of initial activity Vibrio sp. W13
Cu2+ 1 mM, 81% of initial activity Vibrio sp. W13
Mn2+ 1 mM, 75% of initial activity Vibrio sp. W13
Zn2+ 1 mM, 66% of initial activity Vibrio sp. W13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) Km value 0.50 mg/ml, pH 8.0, 30°C Vibrio sp. W13
additional information
-
 alginate Km value 0.67 mg/ml, pH 8.0, 30°C Vibrio sp. W13
additional information
-
 poly(alpha-(1,4)-L-guluronate) Km value 1.04 mg/ml, pH 8.0, 30°C Vibrio sp. W13
additional information
-
 poly(beta-(1,4)-D-mannuronate) Km value 6.9 mg/ml, pH 8.0, 30°C Vibrio sp. W13

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 135% of initial activity Vibrio sp. W13
Co2+ 1 mM, 156% of initial activity Vibrio sp. W13
Fe2+ 1 mM, 148% of initial activity Vibrio sp. W13
Na+ optimum concentration 0.3 M, about 2fold increase in activity compared to absence of NaCl Vibrio sp. W13

Organism

Organism UniProt Comment Textmining
Vibrio sp. W13 A0A0A1DYR7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified by Ni-NTA Sepharose affinity chromatography Vibrio sp. W13

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
457
-
 pH 8.0, 30°C Vibrio sp. W13

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Vibrio sp. W13 unsaturated algino-oligosaccharides
-
 ?
additional information AlgB mainly releases oligosaccharides with a degree of polymersiation of 2-5 from the different kinds of substrates in an endolytic manner Vibrio sp. W13 ?
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Vibrio sp. W13 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate)
-
 Vibrio sp. W13 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate)
-
 Vibrio sp. W13 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 54120, calculated from sequence, x * 55050, SDS-PAGE, mature enzyme Vibrio sp. W13

Synonyms

Synonyms Comment Organism
AlgB
-
 Vibrio sp. W13

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Vibrio sp. W13

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, about 60% residual activity Vibrio sp. W13

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Vibrio sp. W13

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio sp. W13
-
-
 4.9