Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | slightly activating at 0.001%, slightly inhibitory at 0.01% | Saccharophagus sp. Myt-1 | |
Tween 20 | slightly activating at 0.001-0.01% | Saccharophagus sp. Myt-1 | |
Tween 40 | slightly activating at 0.001-0.01% | Saccharophagus sp. Myt-1 | |
Tween 80 | slightly activating at 0.001-0.01% | Saccharophagus sp. Myt-1 |
Cloned (Comment) | Organism |
---|---|
gene algMytC, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) partly in inclusion bodies | Saccharophagus sp. Myt-1 |
General Stability | Organism |
---|---|
activity of AlgMytC is stable in the presence of various detergents | Saccharophagus sp. Myt-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibition at 1-5 mM | Saccharophagus sp. Myt-1 | |
EDTA | - |
Saccharophagus sp. Myt-1 | |
Mg2+ | inhibition at 1-5 mM | Saccharophagus sp. Myt-1 | |
additional information | activity of AlgMytC is stable in the presence of various detergents | Saccharophagus sp. Myt-1 | |
Triton X-100 | slightly activating at 0.001%, slightly inhibitory at 0.01% | Saccharophagus sp. Myt-1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | a metal ion is required. No or poor effects by SDS at 0.001-0.01%, and DTT, Na+ and K+ at 1-5 mM | Saccharophagus sp. Myt-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37573 | - |
x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Saccharophagus sp. Myt-1 |
43000 | - |
x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Saccharophagus sp. Myt-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharophagus sp. Myt-1 | L8B3Z6 | gene algMytC | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble and renatured His-tagged enzyme from Escherichia coli strain BL21(DE3) | Saccharophagus sp. Myt-1 |
Renatured (Comment) | Organism |
---|---|
solubilization of enzyme from inclusion bodies by 8 M urea in Tris-HCl, pH 8.0 | Saccharophagus sp. Myt-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | AlgMytC is predicted to preferably degrade guluronate-blocks because of the presence of the QIH motif in the conserved signature of the amino acid sequence | Saccharophagus sp. Myt-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Saccharophagus sp. Myt-1 |
Synonyms | Comment | Organism |
---|---|---|
AlgMytC | - |
Saccharophagus sp. Myt-1 |
alkaliphilic alginate lyase | - |
Saccharophagus sp. Myt-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Saccharophagus sp. Myt-1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | 30% of maximal activity at 15°C and 45°C | Saccharophagus sp. Myt-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | purified recombinant enzyme, pH 9.0, completely stable at 25°C for 24 h and at 35°C for 1 h, but loss of 60% activity after 24 h at 35°C | Saccharophagus sp. Myt-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Saccharophagus sp. Myt-1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 90% of maximal activity at pH 8.5 and pH 10.0, 30% at pH 7.5, inactive below, profile overview | Saccharophagus sp. Myt-1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | purified recombinant enzyme, 25°C, 24 h, over 70% activity remaining | Saccharophagus sp. Myt-1 |
8.5 | 10 | purified recombinant enzyme, 25°C, 24 h, over 90% activity remaining | Saccharophagus sp. Myt-1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharophagus sp. Myt-1 | sequence calculation | - |
6.6 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme contains the conserved amino acid sequences RTELREM, QIH, and YFKAGVYNQ of the polysaccharide lyase family 7 | Saccharophagus sp. Myt-1 |