Any feedback?
Literature summary for 4.2.2.11 extracted from
- Characterization of a new alginate lyase from newly isolated Flavobacterium sp. S20 (2013), J. Ind. Microbiol. Biotechnol., 40, 113-122.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of renewable biofuels | Flavobacterium sp. |
| synthesis | alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of biochemicals. Alg2A can be a good tool for the large-scale preparation of alginate oligosaccharides with high degree of polymerization | Flavobacterium sp. |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Flavobacterium sp. |
| gene alg2A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Flavobacterium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 66% inhibition | Flavobacterium sp. |  |
| Co2+ | 5 mM, almost complete inhibition | Flavobacterium sp. | |
| Cu2+ | 5 mM, almost complete inhibition | Flavobacterium sp. | |
| Fe2+ | 5 mM, almost complete inhibition | Flavobacterium sp. | |
| Mg2+ | 5 mM, 66% inhibition | Flavobacterium sp. | |
| Mn2+ | 5 mM, almost complete inhibition | Flavobacterium sp. | |
| Zn2+ | 5 mM, almost complete inhibition | Flavobacterium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Flavobacterium sp. | |
| additional information | - |
alginate | Km value 3.33 mg/ml, pH 8.5, 30°C | Flavobacterium sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Flavobacterium sp. | |
| K+ | 5 mM, 148% of initial activity | Flavobacterium sp. | |
| Na+ | activates | Flavobacterium sp. | |
| Na+ | 5 mM, 119% of initial activity | Flavobacterium sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Flavobacterium sp. | Alg2A has a different endolytic reaction mode from both the two commercial alginate lyases and other alginate lyases from polysaccharide lyase family 7 owing to high yields of penta-, hex-, and hepta-saccharides in the hydrolysis products of Alg2A | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium sp. | G9CHX6 | - |
- |
| Flavobacterium sp. | G9CHX6 | gene alg2A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 5.74fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Flavobacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 265.4 | - |
purified recombinant His-tagged enzyme, pH 8.5, 45°C | Flavobacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Flavobacterium sp. | ? | high yields of penta-, hex-, and heptasaccharides in the hydrolysis products | ? | |
| additional information | Alg2A has a different endolytic reaction mode from both the two commercial alginate lyases and other alginate lyases from polysaccharide lyase family 7 owing to high yields of penta-, hex-, and hepta-saccharides in the hydrolysis products of Alg2A | Flavobacterium sp. | ? | - |
? | |
| additional information | the enzyme shows low activity with poly(beta-D-mannuronate), reaction of EC 4.2.2.3 | Flavobacterium sp. | ? | - |
? | |
| poly(alpha-(1->4)-L-guluronic acid) | preferred over poly-(beta1,4-D-mannuronan) | Flavobacterium sp. | ? | - |
? | |
| poly-(alpha-L-guluronate) | - |
Flavobacterium sp. | ? | - |
? | |
| poly-(beta-(1->4)-D-mannuronan) | - |
Flavobacterium sp. | ? | - |
? | |
| sodium alginate | Alg2A prefers poly-(alpha-L-guluronate) as a substrate over poly-(beta-D-mannuronate), substrate specificity, overview | Flavobacterium sp. | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alg2A | - |
Flavobacterium sp. |
| alginate lyase | - |
Flavobacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Flavobacterium sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 40% of maximal activity at 20°C and 70°C, profile overview | Flavobacterium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable below | Flavobacterium sp. |
| 45 | - |
purified recombinant His-tagged enzyme, pH 8.5, 1 h, stable up to | Flavobacterium sp. |
| 50 | - |
purified recombinant His-tagged enzyme, pH 8.5, 1 h, inactivation | Flavobacterium sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 872.8 | - |
sodium alginate | recombinant His-tagged enzyme, pH 8.5, 45°C | Flavobacterium sp. | |
| 873 | 999 | alginate | pH 8.5, 30°C | Flavobacterium sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Flavobacterium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | more than 60% of maximum activity within | Flavobacterium sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
24 h, 20% residual activity | Flavobacterium sp. |
| 4 | 10 | purified recombinant His-tagged enzyme, 45°C, the enzyme retains over 60% activity after 24 h incubation at pH 5.0-10.0, but loses about 80% activity at pH 4.0 after 24 h | Flavobacterium sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Alg2A belongs to the polysaccharide lyase family 7, but has a different endolytic reaction mode from other alginate lyases from polysaccharide lyase family 7 owing to high yields of penta-, hex-, and hepta-saccharides in the hydrolysis products of Alg2A, overview | Flavobacterium sp. |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
alginate | kcat/Km value 26 ml/mg/s, pH 8.5, 30°C | Flavobacterium sp. | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
