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Literature summary for 4.2.2.11 extracted from

  • Wang, Y.; Guo, E.W.; Yu, W.G.; Han, F.
    Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp. (2013), Biotechnol. Lett., 35, 703-708.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 47% residual activity Vibrio sp.
AlCl3 53% inhibition at 1 mM Vibrio sp.
BaCl2 28% inhibition at 1 mM Vibrio sp.
Cu2+ 1 mM, 3% residual activity Vibrio sp.
CuCl2 97% inhibition at 1 mM Vibrio sp.
EDTA 1 mM, 6% residual activity; 96% inhibition at 1 mM Vibrio sp.
Fe3+ 1 mM, 8% residual activity Vibrio sp.
FeCl3 92% inhibition at 1 mM Vibrio sp.
NiCl2 33% inhibition at 1 mM Vibrio sp.
Zn2+ 1 mM, 42% residual activity Vibrio sp.
ZnCl2 58% inhibition at 1 mM Vibrio sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Vibrio sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 120% of initial activity Vibrio sp.
CaCl2 20% activation at 1 mM Vibrio sp.
Mg2+ 1 mM, 115% of initial activity Vibrio sp.
MgCl2 15% activation at 1 mM Vibrio sp.
Mn2+ 1 mM, 112% of initial activity Vibrio sp.
MnCl2 12% activation at 1 mM Vibrio sp.
additional information no effect on enzyme activity by SDS, K+, Li+, and NH4+ Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-
Vibrio sp.
-
QY105
-
Vibrio sp. QY105
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 23.6fold from cell culture supernatant Vibrio sp.
purification from culture liquid Vibrio sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
Vibrio sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2152
-
pH 7.0, 38°C Vibrio sp.
2152
-
purified enzyme, pH 7.0, 38°C Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
Vibrio sp. ?
-
?
alginate
-
Vibrio sp. QY105 ?
-
?
additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. ?
-
?
additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. QY105 ?
-
?
poly(alpha-(1->4)-L-guluronic acid) 108% of the activity with alginate Vibrio sp. ? main products are disaccharide, trisaccharide and tetrasaccharide ?
poly(alpha-L-guluronate) best substrate Vibrio sp. ?
-
?
poly-(beta-(1->4)-D-mannuronan) 30% of the activity with alginate Vibrio sp. ? main products are trisaccharide, tetrasaccharide and pentasaccharide ?
sodium alginate high activity Vibrio sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Vibrio sp.

Synonyms

Synonyms Comment Organism
alginate lyase
-
Vibrio sp.
AlyV5
-
Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
10 min, 40% residual activity Vibrio sp.
90
-
purified native enzyme, 10 min, pH 7.0, 40% activity remains Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
Vibrio sp.
6 9 purified native enzyme, 40°C, stable within this pH range Vibrio sp.