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Literature summary for 4.2.2.11 extracted from
- Cloning and characterization of a novel oligoalginate lyase from a newly isolated bacterium Sphingomonas sp. MJ-3 (2012), Mar. Biotechnol., 14, 189-202.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sphingomonas sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
| 79900 | - |
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
| 80000 | - |
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas sp. | G1EH67 | - |
- |
| Sphingomonas sp. MJ-3 | G1EH67 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Sphingomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-guluronosyl linkage in alginate | - |
Sphingomonas sp. | ? | final degradation products are alginate monosaccharides | ? |  |
| alpha-L-guluronosyl linkage in alginate | - |
Sphingomonas sp. MJ-3 | ? | final degradation products are alginate monosaccharides | ? | |
| poly(alpha-(1->4)-L-guluronic acid) | 90% of the activity with alginate | Sphingomonas sp. | ? | - |
? | |
| poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) | alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. 120% of the activity with alginate | Sphingomonas sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE | Sphingomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sphingomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Sphingomonas sp. |
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