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Literature summary for 4.2.2.11 extracted from
- Production and characterization of guluronate lyase from Klebsiella pneumoniae for applications in seaweed biotechnology (1993), Enzyme Microb. Technol., 15, 756-763.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alginates with low guluronic content | cultures in the presence of alginates with low guluronic content give reduced biomass but favor enzyme production | Klebsiella pneumoniae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is lost at standard sea water salinity, restored in the presence of 1 mM calcium, activity is lost even at low salt concentrations if calcium is removed by a calcium chelator | Klebsiella pneumoniae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Klebsiella pneumoniae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | essential cofactor, not only affects enzyme kinetics but also the final degree of conversion | Klebsiella pneumoniae | |
| Na+ | concentrations higher than 3% w/w produce complete loss of activity, the activity is partially restored with 1 mM calcium | Klebsiella pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly-alpha-L-guluronic acid | Klebsiella pneumoniae | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| cell-free extract at -20 °C | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-guluronosyl linkage in alginate | significant activity is found not only on guluronate-guluronate linkages but also on guluronate-mannuronate linkages | Klebsiella pneumoniae | ? | - |
? | |
| poly-alpha-L-guluronic acid | - |
Klebsiella pneumoniae | ? | - |
? | |
| poly-alpha-L-guluronic acid | - |
Klebsiella pneumoniae | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
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