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Literature summary for 4.2.2.11 extracted from

  • Nibu, Y.; Satoh, T.; Nishi, Y.; Takeuchi, T.; Murata, K.; Kusakabe, I.
    Purification and characterization of extracellular alginate lyase from Enterobacter cloacae M-1 (1995), Biosci. Biotechnol. Biochem., 59, 632-637.
    View publication on PubMed
Search for more literature for 4.2.2.11

General Stability

General Stability Organism
unstable to heating, shows low activity when mantained in 50 mM Tris-HCl buffer Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, approximately 95% inhibition, enzymatic activity is restored totally by treatment with calcium chloride or partially restored with aluminium chloride or manganese (II) chloride Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Enterobacter cloacae
38000
-
 1 * 38000, SDS-PAGE Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
 strain M-1,intracellular and extracellular enzyme
-
Enterobacter cloacae M-1intracellular
-
 strain M-1,intracellular and extracellular enzyme
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, cation exchange chromatography and gel filtration Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-guluronosyl linkage in alginate endolyase Enterobacter cloacae ?
-
 ?
alpha-L-guluronosyl linkage in alginate significant activity is found not only on guluronate-guluronate linkages but also on guluronate-mannuronate linkages Enterobacter cloacae ?
-
 ?
alpha-L-guluronosyl linkage in alginate endolyase Enterobacter cloacae M-1intracellular ?
-
 ?
alpha-L-guluronosyl linkage in alginate significant activity is found not only on guluronate-guluronate linkages but also on guluronate-mannuronate linkages Enterobacter cloacae M-1intracellular ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Enterobacter cloacae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Enterobacter cloacae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 no activity is measured after treatment at this temperature, activity decreases with increasing temperature Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Enterobacter cloacae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable Enterobacter cloacae

pI Value

Organism Comment pI Value Maximum pI Value
Enterobacter cloacae
-
-
 8.9