Application | Comment | Organism |
---|---|---|
synthesis | maximal production of functional Pnl1 is obtained after induction by 0.4 mM isopropyl beta-D-thiogalactoside at 30°C and 150 rpm for 16 h. The use of Origami host strain significantly improves the yield of the soluble active form of the enzyme | Penicillium sp. 'occitanis' |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Penicillium sp. 'occitanis' |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium sp. 'occitanis' | Q0PH38 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, single step purification using His-Trap affinity column chromatography | Penicillium sp. 'occitanis' |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Penicillium sp. 'occitanis' |
Synonyms | Comment | Organism |
---|---|---|
Pnl1 | - |
Penicillium sp. 'occitanis' |