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Literature summary for 4.2.2.10 extracted from
- Purification and characterisation of thermo-alkaline pectinase enzyme from Hylocereus polyrhizus (2014), Eur. Food Res. Technol., 239, 21-29.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-cystine | 5 mM, 142% of initial activity | Hylocereus polyrhizus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 10 mM, 5% residual activity | Hylocereus polyrhizus | |
| Fe2+ | 10 mM, no residual activity | Hylocereus polyrhizus | |
| N-bromosuccinimide | 5 mM, no residual activity | Hylocereus polyrhizus | |
| Ni2+ | 10 mM, 24% residual activity | Hylocereus polyrhizus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
polygalacturonic acid | Km value 2.7 mg/ml, pH 8.0, 75°C | Hylocereus polyrhizus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 120% of initial activity | Hylocereus polyrhizus | |
| Mg2+ | 10 mM, 112% of initial activity | Hylocereus polyrhizus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Hylocereus polyrhizus |
| 34200 | - |
1 * 34200, SDS-PAGE | Hylocereus polyrhizus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hylocereus polyrhizus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hylocereus polyrhizus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside | - |
Hylocereus polyrhizus | ? | - |
? | |
| arabinan | - |
Hylocereus polyrhizus | ? | - |
? | |
| oat spelt xylan | - |
Hylocereus polyrhizus | ? | - |
? | |
| polygalacturonic acid | - |
Hylocereus polyrhizus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34200, SDS-PAGE | Hylocereus polyrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Hylocereus polyrhizus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 8 | - |
stable for 1 h | Hylocereus polyrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Hylocereus polyrhizus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | - |
Hylocereus polyrhizus |
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