Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.10 extracted from

  • Pedrolli, D.B.; Carmona, E.C.
    Pectin lyase from Aspergillus giganteus: comparative study of productivity of submerged fermentation on citrus pectin and orange waste (2010), Prikl. Biokhim. Mikrobiol., 45, 677-683.
    View publication on PubMed
Search for more literature for 4.2.2.10

Application

Application Comment Organism
synthesis production of pectin lyase by Aspergillus giganteus. The highest activity, using citrus pectin as carbon source, is obtained in 11-day-old standing cultures, but the highest specific activity is obtained in 6.5-day-old shaken cultures, at pH 6.5 and 35°C. Using orange waste as carbon source, the highest activity is observed in 8-day-old standing cultures, at pH 7.0 and 30°C Aspergillus giganteus

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apple pectin
-
 Aspergillus giganteus ?
-
 ?
citrus pectin citrus pectin, with 72% of methylation, best substrate Aspergillus giganteus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus giganteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 pH-range 6.0-10, 24 h, more than 85% of initial activity Aspergillus giganteus
45
-
 half-life 30 min Aspergillus giganteus
50
-
 half-life 27 min Aspergillus giganteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 enzyme from culture in orange waste Aspergillus giganteus
9
-
 enzyme from culture in citrus pectin Aspergillus giganteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 room temperature, 24 h, more than 85% of initial activity Aspergillus giganteus