Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium arsenate | slightly promotes the activity of PNL | Aspergillus terricola |
Application | Comment | Organism |
---|---|---|
additional information | the purified pectin lyase shows retting ability for natural fibers viz. Cannabis sativa and Linum usitatissimum | Aspergillus terricola |
General Stability | Organism |
---|---|
the purified enzyme retains its full activity after exposure for 1 h at 60°C in 1.0 M ammonium sulfate, in the absence of ammonium sulfate the enzyme loses its 77% activity at 60°C, at higher temperatures (70-100°C), ammonium sulfate is not able to stabilize the activity of the pectin lyase | Aspergillus terricola |
the purified enzyme retains its full activity after exposure for 1 h at 60°C in 1.0 M ammonium sulfate, in the absence of ammonium sulfate, the enzyme loses its 77% activity at 60°C, at higher temperatures (70-100°C), ammonium sulfate is not able to stabilize the activity of the pectin lyase | Aspergillus terricola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Aspergillus terricola | |
Cu2+ | 10% residual activity at 1 mM | Aspergillus terricola | |
EDTA | 85% residual activity at 1 mM | Aspergillus terricola | |
Hg2+ | 24% residual activity at 1 mM | Aspergillus terricola | |
K+ | 91% residual activity at 1 mM; 92% residual activity at 1 mM | Aspergillus terricola | |
K4[Fe(CN)6] | complete inhibition at 1 mM | Aspergillus terricola | |
KMnO4 | complete inhibition at 1 mM | Aspergillus terricola | |
Mg2+ | 97% residual activity at 1 mM | Aspergillus terricola | |
Mn2+ | 93% residual activity at 1 mM | Aspergillus terricola | |
potassium ferrocyanide | complete inhibition at 1 mM | Aspergillus terricola | |
potassium permanganate | complete inhibition at 1 mM | Aspergillus terricola | |
Sodium azide | 79% residual activity at 1 mM | Aspergillus terricola | |
Zn2+ | 69% residual activity at 1 mM | Aspergillus terricola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly promotes the activity of PNL (105% relative activity at 1 mM) | Aspergillus terricola | |
Ca2+ | 105% relative activity at 1 mM | Aspergillus terricola | |
Co2+ | slightly promotes the activity of PNL (114% relative activity at 1 mM) | Aspergillus terricola | |
Co2+ | 114% relative activity at 1 mM | Aspergillus terricola | |
Na+ | slightly promotes the activity of PNL (108.3% relative activity at 1 mM) | Aspergillus terricola | |
Sodium arsenate | slightly promotes the activity of PNL (110% relative activity at 1 mM) | Aspergillus terricola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Aspergillus terricola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terricola | - |
- |
- |
Aspergillus terricola MTCC 7588 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE cellulose column chromatography and Sephadex G-100 gel filtration | Aspergillus terricola |
DEAE-cellulose column chromatography and Sephadex G100 gel filtration | Aspergillus terricola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin | - |
Aspergillus terricola | ? | - |
? | |
citrus pectin | - |
Aspergillus terricola MTCC 7588 | ? | - |
? | |
additional information | pectin lyase shows retting ability for natural fibers of Cannabis sativa and Linum usitatissimum | Aspergillus terricola | ? | - |
? | |
additional information | pectin lyase shows retting ability for natural fibers of Cannabis sativa and Linum usitatissimum | Aspergillus terricola MTCC 7588 | ? | - |
? | |
pectin | - |
Aspergillus terricola | ? | - |
? | |
pectin | - |
Aspergillus terricola MTCC 7588 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNL | - |
Aspergillus terricola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus terricola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | the enzyme retains its full activity up to 50°C, and thereafter, activity decreases with increase in temperature, 75% of the activity is lost after incubating the purified PNL at 60°C for 1 h, the half-lives at 60, 70, 80, and 85°C are 7.03, 3.9, 1.8, and 0.89 min, respectively | Aspergillus terricola |
50 | 60 | the enzyme retains its full activity up to 50°C, and thereafter, activity decreased with increase in temperature, however 75% of the activity is lost after incubating the purified PNL at 60°C for 1 h | Aspergillus terricola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
citrus pectin | at 37°C | Aspergillus terricola | |
110 | - |
Pectin | purified enzyme, at 37°C | Aspergillus terricola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aspergillus terricola |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Aspergillus terricola |
4 | 9 | the enzyme retains approximately 100% of its maximum activity for 24 h in a buffer of pH range 4.0-9.0 | Aspergillus terricola |