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Literature summary for 4.2.2.10 extracted from
- Studies on pectin lyase (1975), Mededelingen Landbouwhogeschool, Communications Agricultural University, 75-13, 1-100.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| high ionic strength or high salt concentration appear to be essential for enzyme stability | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | - |
Aspergillus niger |  |
| formate | - |
Aspergillus niger | |
| iso-butyrate | - |
Aspergillus niger | |
| n-Butyrate | - |
Aspergillus niger | |
| propionate | - |
Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus niger |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimal concentration above pH 4.5: 50 mM. Optimal cocentration below pH 4.5: 30 mM | Aspergillus niger | |
| Na+ | optimal concentration above pH 4.5: 300 mM. Optimal concentration below pH 4.5: 100 mM | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35400 | 36800 | type II enzyme, gel filtration | Aspergillus niger |
| 35500 | 37950 | type I enzyme, gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
pectin lyase type I and type II | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | 2.5% sugar content, based on weight. Type I enzyme contains only mannose residues, 4 mol/mol. Type II enzyme can be separated into two species which are different in their ratio of mannose to glucose residues | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | - |
Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45050-50150, enzyme type I, SDS-PAGE | Aspergillus niger |
| monomer | 1 * 43800-49450, enzyme type II, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ultrazym-100 | Ciba-Geigy | Aspergillus niger |
| Ultrazym-20 | Ciba-Geigy | Aspergillus niger |
| Ultrazym-40 | Ciba-Geigy | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
a bell shaped pH profile at low substrate concentrations, shifts of the pH optimum to more basic values upon increasing substrate concentrations accompanied by deviation of the profile from a bell shaped curve | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
inactivation above pH 6 | Aspergillus niger |
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Release 2023.1 (January 2023)
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