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Literature summary for 4.2.2.10 extracted from

  • Wijesundera, R.L.C.; Bailey, J.A.; Byrde, R.J.W.
    Production of pectin lyase by Colletotrichum lindemuthianum in culture and in infected bean (Phaseolus vulgaris) tissue (1984), J. Gen. Microbiol., 130, 285-290.
No PubMed abstract available
Search for more literature for 4.2.2.10

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity increases with the increase in Ca2+ concentration Colletotrichum lindemuthianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000 23500 enzyme form PL I, gel filtration Colletotrichum lindemuthianum
27000 28500 enzyme form PL II, gel filtration Colletotrichum lindemuthianum

Organism

Organism UniProt Comment Textmining
Colletotrichum lindemuthianum
-
 2 enzyme forms: PLI, PLII
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture in medium containing sodium polypectate or cell walls from Phaseolus vulgaris Colletotrichum lindemuthianum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin
-
 Colletotrichum lindemuthianum ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, 75% loss of activity Colletotrichum lindemuthianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 enzyme form PL I, sodium polypectate medium Colletotrichum lindemuthianum
9.3
-
 enzyme form PL I, cell wall medium Colletotrichum lindemuthianum
9.8
-
 enzyme form PL II, sodium polypectate medium or cell wall medium Colletotrichum lindemuthianum

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 pH 8.5: about 30% of maximal activity, pH 10: about 60% of maximal activity, enzyme form PL I, polypectate culture filtrate Colletotrichum lindemuthianum
8.5 10.5 pH 8.5: about 50% of maximal activity, pH 10.5: about 40% of maximal activity, enzyme form PL II, polypectate culture filtrate Colletotrichum lindemuthianum