Protein Variants | Comment | Organism |
---|---|---|
M179V/M181V | mutations in the hot spot region of HylP, lead to fibrillation with the seeding of prefibrils | Streptococcus pyogenes phage |
additional information | N-terminal swapped chimeras between phage hyaluronan lyases HylP and HylP2, i.e. N-HylP2-C-HylP and N-HylP-C-HylP2 or HylP do not form fibrils at acidic pH. Seeding of prefibrils of HylP2 recompenses nucleation and leads to fibrillation in N-HylP-C-HylP2 | Streptococcus pyogenes phage |
V147A | mutation in the hot spot region, abolishes fibril formation in HylP2 | Streptococcus pyogenes phage |
Organism | UniProt | Comment | Textmining |
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Streptococcus pyogenes phage | - |
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General Information | Comment | Organism |
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physiological function | presence of a hot spot region (A144GVVVY149) towards the carboxy terminus of isoform HylP2, which is essential for the acceleration of fibril formation. The hot spot is inherently mutated for valines (A178AMVMY183) in case of isoform HylP. The N-terminal swapped chimeras between these phage hyaluronan lyases (N-HylP2-C-HylP and N-HylP-C-HylP2) or HylP do not form fibrils at acidic pH. Seeding of prefibrils of HylP2 recompenses nucleation and leads to fibrillation in N-HylP-C-HylP2. The V147A mutation in the hot spot region abolishes fibril formation in HylP2. The M179V and M181V double mutations in the hot spot region of HylP leads to fibrillation with the seeding of prefibrils. Fibrillation in isoform HylP2 is initiated by the N-terminus, but may be accelerated by the conserved hot spot region in the C-terminus. A collagenous (Gly-X-Y)10 motif in the N-terminus and a mutated hot spot region in the C-terminus of isoform HylP affect fibrillar nucleation and acceleration, respectively | Streptococcus pyogenes phage |