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Literature summary for 4.2.2.1 extracted from

  • Shukla, H.; Singh, S.; Singh, A.; Mitra, K.; Akhtar, M.
    The C-terminus hot spot region helps in the fibril formation of bacteriophage-associated hyaluronate lyase (HylP2) (2015), Sci. Rep., 5, 14429 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
M179V/M181V mutations in the hot spot region of HylP, lead to fibrillation with the seeding of prefibrils Streptococcus pyogenes phage
additional information N-terminal swapped chimeras between phage hyaluronan lyases HylP and HylP2, i.e. N-HylP2-C-HylP and N-HylP-C-HylP2 or HylP do not form fibrils at acidic pH. Seeding of prefibrils of HylP2 recompenses nucleation and leads to fibrillation in N-HylP-C-HylP2 Streptococcus pyogenes phage
V147A mutation in the hot spot region, abolishes fibril formation in HylP2 Streptococcus pyogenes phage

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes phage
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General Information

General Information Comment Organism
physiological function presence of a hot spot region (A144GVVVY149) towards the carboxy terminus of isoform HylP2, which is essential for the acceleration of fibril formation. The hot spot is inherently mutated for valines (A178AMVMY183) in case of isoform HylP. The N-terminal swapped chimeras between these phage hyaluronan lyases (N-HylP2-C-HylP and N-HylP-C-HylP2) or HylP do not form fibrils at acidic pH. Seeding of prefibrils of HylP2 recompenses nucleation and leads to fibrillation in N-HylP-C-HylP2. The V147A mutation in the hot spot region abolishes fibril formation in HylP2. The M179V and M181V double mutations in the hot spot region of HylP leads to fibrillation with the seeding of prefibrils. Fibrillation in isoform HylP2 is initiated by the N-terminus, but may be accelerated by the conserved hot spot region in the C-terminus. A collagenous (Gly-X-Y)10 motif in the N-terminus and a mutated hot spot region in the C-terminus of isoform HylP affect fibrillar nucleation and acceleration, respectively Streptococcus pyogenes phage