Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.1 extracted from

  • Kurata, A.; Matsumoto, M.; Kobayashi, T.; Deguchi, S.; Kishimoto, N.
    Hyaluronate lyase of a deep-sea Bacillus niacini (2015), Mar. Biotechnol., 17, 277-284 .
    View publication on PubMed
Search for more literature for 4.2.2.1

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzylbromide 2.5 mM, 87.4% inhibition Neobacillus niacini
dithiothreitol 5 mM, 30% inhibition Neobacillus niacini
iodoacetate 5 mM, slight inhibition Neobacillus niacini
N-bromosuccinimide 5 mM, slight inhibition Neobacillus niacini
N-ethylmaleimide 5 mM, slight inhibition Neobacillus niacini
p-chloromercuribenzoate 5 mM, 30% inhibition Neobacillus niacini

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Neobacillus niacini
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ optimum concentrations are 5, 50, and 100 mM calcium ions for degradation of hyaluronan, chondroitin sulfate C, and chondroitin sulfate A, respectively Neobacillus niacini
Mn2+ 82% of the activity with Ca2+ Neobacillus niacini
additional information less than 20% of maximum activity in absence of metal ions Neobacillus niacini
Sr2+ 78.6% of the activity with Ca2+ Neobacillus niacini

Organism

Organism UniProt Comment Textmining
Neobacillus niacini X5I0R2
-
-
Neobacillus niacini JAM F8 X5I0R2
-
-

Purification (Commentary)

Purification (Comment) Organism
from a culture broth Neobacillus niacini

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
136.7
-
 substrate hyaluronan, pH 6.0, 37°C Neobacillus niacini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A 44% of the activity with hyaluronan Neobacillus niacini ?
-
 ?
chondroitin sulfate A 44% of the activity with hyaluronan Neobacillus niacini JAM F8 ?
-
 ?
chondroitin sulfate C 30% of the activity with hyaluronan Neobacillus niacini 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-galactose
-
 ?
chondroitin sulfate C 30% of the activity with hyaluronan Neobacillus niacini JAM F8 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-galactose
-
 ?
hyaluronan enzyme displays endo-type degratation. The viscosity of hyaluronan decreases to 37% of the original one after 3-min incubation Neobacillus niacini 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-glucose
-
 ?
hyaluronan enzyme displays endo-type degratation. The viscosity of hyaluronan decreases to 37% of the original one after 3-min incubation Neobacillus niacini JAM F8 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-glucose
-
 ?
additional information chondroitin, 2.3% of the activity with hyaluronan Neobacillus niacini ?
-
 ?
additional information chondroitin, 2.3% of the activity with hyaluronan Neobacillus niacini JAM F8 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Neobacillus niacini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Neobacillus niacini

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 10% of maximum activity Neobacillus niacini

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, absence of Ca2+, 30% residual lactivity Neobacillus niacini
45
-
 15 min, stable in presence of Ca2+ Neobacillus niacini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Neobacillus niacini

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 stable in presence of Ca2+ Neobacillus niacini
6 10 stable in absence of Ca2+ Neobacillus niacini